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| DOI: | 10.3852/mycologia.98.3.410 |
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| Web URL(s): | http://www.mycologia.org/content/98/3/410.full
Last checked: 07/16/2013
Access conditions: Item is within a limited-access website
http://www.mycologia.org/content/98/3/410.full.pdf
Last checked: 07/16/2013
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Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Villa, Neilyn O.;
Kageyama, Koji;
Asano, Takahiro;
Suga, Haruhisa |
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| Author Affiliation: | Villa: Genetics and Molecular Biology Division, Institute of Biological Sciences, University of the Philippines Los Baños, College, Laguna, Philippines; Kageyama and Asano: River Basin Research Center; Suga: Life Science Research Center, Gifu University, Yanagido, Japan |
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| Title: | Phylogenetic relationships of Pythium and Phytophthora species based on ITS rDNA, cytochrome oxidase II and β-tubulin gene sequences |
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| Section: | Molecular evolution and systematics
Other records with the "Molecular evolution and systematics" Section
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| Source: | Mycologia. Vol. 98, No. 3, May/June 2006, p. 410-422. |
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| Publishing Information: | Lancaster, Pennsylvania: New Era Print Co. for the New York Botanical Garden |
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| # of Pages: | 13 |
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| Related Web URL: | http://www.mycologia.org/content/98/3/410.abstract
Last checked: 07/16/2013
Notes: Abstract only |
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| Keywords: | TIC Keywords: Analytical methods; Comparisons; Cytochrome c oxidase; DNA; Genetic analysis; Morphological evaluation; Morphology; Mycology; Oomycota; Phylogenetic analysis; Phytophthora; Polymerase chain reaction; Pythium; Sporangia
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| Abstract/Contents: | "Fifty-eight isolates representing 39 Pythium species and 17 isolates representing nine Phytophthora species were chosen to investigate intra- and intergeneric relationships with sequence analysis of three genomic areas. The internal transcribed spacer regions (ITS1 and ITS2), including the 5.8S gene of the ribosomal DNA were PCR amplified with the universal primers ITS1 and ITS4. On the other hand 563 bp of the cytochrome oxidase II (cox II) gene was amplified with the primer pair FM66 and FM58 for Pythium and FM75 and FM78 for Phytophthora. The 658 bp partial β-tubulin gene was amplified with the forward primer BT5 and reverse primer BT6. Maximum parsimony analysis of the three DNA regions revealed four major clades, reflective of sporangial morphology. Clade 1 was composed of Pythium isolates that bear filamentous to lobulate sporangia. Clade 2 represents Pythium isolates that bear globose to spherical zoosporangia or spherical hyphal swellings. Meanwhile Phytophthora isolates were lumped into Clade 3 wherein the papillate, semipapillate and nonpapillate species occupied separate subclades. Lastly, Clade 4 was composed of Pythium species that bear subglobose sporangia resembling the papillate sporangia observed in Phytophthora. Hence a number of species (Ph. undulate, P. helicoides,P. ostracodes, P. oedochilum and P. vexans) have been proposed to be the elusive intermediate species in the Pythium-to-Phytophthora evolutionary line." |
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| Language: | English |
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| References: | 44 |
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| Note: | Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Villa, N. O., K. Kageyama, T. Asano, and H. Suga. 2006. Phylogenetic relationships of Pythium and Phytophthora species based on ITS rDNA, cytochrome oxidase II and β-tubulin gene sequences. Mycologia. 98(3):p. 410-422. |
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| DOI: 10.3852/mycologia.98.3.410 |
| Web URL(s):
http://www.mycologia.org/content/98/3/410.full
Last checked: 07/16/2013
Access conditions: Item is within a limited-access website
http://www.mycologia.org/content/98/3/410.full.pdf
Last checked: 07/16/2013
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: b2214983
MSU catalog number: b5343430 |
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