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| Web URL(s): | http://www.swss.ws/wp-content/uploads/docs/2008 Proceedings-SWSS.pdf#page=167
Last checked: 07/24/2013
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Author(s): | Cutulle, M. A.;
McElroy, J. S.;
Stewart, C. N. Jr.;
Yuan, J. S. |
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| Author Affiliation: | Department of Plant Science, The University of Tennessee, Knoxville, TN |
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| Title: | PCR-based assay for rapid detection of mutations associated with dinitroaniline resistant annual bluegrass |
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| Section: | Weed management in turf
Other records with the "Weed management in turf" Section
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| Meeting Info.: | Jacksonville, Florida: January 27-30, 2008 |
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| Source: | 2008 Proceedings, Southern Weed Science Society. Vol. 61, 2008, p. 98. |
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| Publishing Information: | Champaign, Illinois: Southern Weed Science Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Bioassay; Dinitroaniline herbicides; Herbicide resistance; Mutations; Poa annua; Polymerase chain reaction
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| Abstract/Contents: | "A suspected dinitroaniline herbicide-resistant annual bluegrass ecotype (Poa annua L.) was harvested from Eagle Bluff golf course in Chattanooga, TN. Herbicide Bioassays compared the growth response of the Chattanooga population with a sensitive control population. Growth response curves indicated that the Chattanooga population was resistant to dinitroanilines. Bioassays usually require mature seed and can be very labor intensive. Nucleic acid based screens do not require living tissue and the diagnosis time is short. Therefore, elucidation of the mutation conferring resistance to dinitroanilines in the Chattanooga annual bluegrass should be obtained. Subsequently, a PCR assay which selects for the mutation may be performed for a quick diagnosis. A single base pair mutation in the α-tubulin gene changing threonine to isoleucine has been shown to confer resistance to dinitroanilines in multiple weeds. Genomic DNA was extracted from the Chattanooga population and the sensitive control. The α-tubulin gene was sequenced from both populations. Sequences from both populations were aligned with the α-tubulin from a dinitroaniline resistant green foxtail (Setaria viridais). Alignment revealed that a C-T point mutation changing threonine to isoleucine is present in both dinitroaniline resistant green foxtail and the Chattanooga population. An allele specific reverse primer designed complementary to the C-T point mutation and a forward primer primary which was specific to α-tubulin from both ecotypes were utilized as primers for a PCR reaction. An annealing temperature of 65 C allowed for preferential selection of the dinitroaniline resistant ecotype template." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Cutulle, M. A., J. S. McElroy, C. N. Jr. Stewart, and J. S. Yuan. 2008. PCR-based assay for rapid detection of mutations associated with dinitroaniline resistant annual bluegrass. South. Weed Sci. Soc. Proc. 61:p. 98. |
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| Web URL(s):
http://www.swss.ws/wp-content/uploads/docs/2008 Proceedings-SWSS.pdf#page=167
Last checked: 07/24/2013
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: b2207931 |
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