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| Web URL(s): | http://www.swss.ws/wp-content/uploads/docs/2008 Proceedings-SWSS.pdf#page=200
Last checked: 07/24/2013
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Author(s): | Borst, S. M.;
McElroy, J. S.;
Wilhelm, S. W. |
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| Author Affiliation: | The University of Tennessee, Knoxville, TN |
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| Title: | Phytopathogenic algae response to common turfgrass pesticide micronutrients |
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| Section: | Weed management in turf
Other records with the "Weed management in turf" Section
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| Meeting Info.: | Jacksonville, Florida: January 27-30, 2008 |
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| Source: | 2008 Proceedings, Southern Weed Science Society. Vol. 61, 2008, p. 108. |
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| Publishing Information: | Champaign, Illinois: Southern Weed Science Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Copper sulfate; Cyanobacteria; Micronutrients; Pathogens; Pesticides; Weed control
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| Abstract/Contents: | "Phytopathogenic algae (filamentous cyanobacteria) encroachment is a persistent weed/pathogen problem on golf course putting green. Algae disrupt water infiltration and can compete for light by covering the leaf surface. Chlorothalonil + zinc and mancozeb + copper hydroxide are labeled for the control of cyanobacteria on golf course putting greens. Both chemistries include turfgrass micronutrients for added pesticide efficacy. Copper sulfate is also utilized as an algaecide in aquatic environments such as catch basins and golf course water retention ponds. Individual cyanobacteria species have different tolerances for micronutrients such as Cu and Zn. Research was initiated to isolate and identify common cyanobacteria species to golf course putting greens and evaluate response to Cu and Zn. Research was conducted in Knoxville, TN. Cyanobacteria cultures were taken from Gettysview Country Club, The University of Tennessee golf practice facility, and the East Tennessee Research and Education Center, all located in Knoxville, TN. Isolates were cultured in BG-11 standard growth media. Single species were isolated via plate streaking and separation. Single filamentous isolates were identified via 16s rRNA gene sequencing with specific 16S cyanobacterial primers. 1148 base pair fragments were amplified. Basic Local Alignment Search Tool (BLAST) was utilized to compare sequences. An in vitro response screen utilizing six different concentrations of ZnSO4 (Zn) and CuSO4 (Cu) (0, 0.63, 3.15, 6.3, 9.45, 12.6 μM) were administered to TN algal isolates. Each concentration was added to 30ml of BG-11 liquid media and inoculated with TN algal filaments. Triplicates of each concentration were then harvested at (0, 3, 5, 7, 10) days after inoculation (DAI) utilizing 0.2μm filters. Cells were lysed and chlorophyll levels (μg/L) were measured with a Turner 10AU Fluorimeter. Isolates were grown in 1000 μE of light and 26°C. For simplicity only concentration levels (0, 0.63, 3.15) will be discussed because exceeding levels were similar to 3.15. BLAST search revealed TN algal isolate had a 97% match to and uncultured California grassland cyanobacteria. TN algal isolate had a 94% match to Phormidium murrayi, an arctic filamentous mat forming cyanobacteria. Sequence and taxonomic traits of TN algal isolate relate to Phormidium spp. cyanobacteria. Micronutrient screens identified that both Zn and Cu at 3.14 μM were lethal 10DAI. Both micronutrients at 0.63μM had an increase in growth at 7 and 10DAI. At 0.63μM, Zn isolates contained 0.04μg/L and Cu isolates contained 0.02 μg/L of chlorophyll 10 DAI. Research indicates that TN algal isolates are controlled by both micronutrients at 3.15 μM. Past research has indicated that chlorothalonil + Zn controls creeping bentgrass algae greater than chlorothalonil alone. Further research is needed to determine soil persistence of Cu and Zn in sand-based putting green root zones, tolerance of creeping bentgrass, and consistency of field control with these micronutrients." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Borst, S. M., J. S. McElroy, and S. W. Wilhelm. 2008. Phytopathogenic algae response to common turfgrass pesticide micronutrients. South. Weed Sci. Soc. Proc. 61:p. 108. |
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| Web URL(s):
http://www.swss.ws/wp-content/uploads/docs/2008 Proceedings-SWSS.pdf#page=200
Last checked: 07/24/2013
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: b2207931 |
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