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Web URL(s): | http://www.swss.ws/wp-content/uploads/docs/2011 Proceedings-SWSS.pdf#page=189 Last checked: 08/05/2013 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Report |
Author(s): | Elmore, Matthew T.;
Brosnan, James T.;
Kopsell, Dean A.;
Breeden, Gregory K.;
Mueller, Thomas C. |
Author Affiliation: | University of Tennessee, Knoxville |
Title: | Methods of assessing the activity of three HPPD inhibiting herbicides |
Section: | Weed management in turf Other records with the "Weed management in turf" Section
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Meeting Info.: | San Juan, Puerto Rico: January 24-26, 2011 |
Source: | 2011 Proceedings, Southern Weed Science Society. Vol. 64, 2011, p. 81. |
Publishing Information: | Champaign, Illinois: Southern Weed Science Society |
# of Pages: | 1 |
Keywords: | TIC Keywords: Chlorophyll fluorescence; Color evaluation; Herbicide application; Liquid chromatography; Mesotrione; Pigmentation; Tembotrione; Topramezone; Visual evaluation
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Abstract/Contents: | "Mesotrione, topramezone, and tembotrione are herbicides that inhibit 4-hydroxyphenylpyruvate dioxygenase (HPPD), indirectly inhibiting carotenoid biosynthesis. Weed control following treatment with HPPD inhibitors is commonly evaluated through visual ratings or assessments of chlorophyll fluorescence. Minimal information is available regarding the accuracy of these techniques for estimating carotenoid and chlorophyll pigment concentrations after HPPD inhibiting herbicide treatment. Research was conducted in 2009 to determine the accuracy of visual ratings and chlorophyll fluorescence yield (Fv/Fm) measurements for evaluating changes in carotenoid and chlorophyll pigment concentrations in bermudagrass (Cynodon dactylon L. Pers. cv. 'Riviera') leaf tissue following treatment with mesotrione, topramezone, and tembotrione. In July 2009, mature bermudagrass plants were transferred to 10 cm pots in a glasshouse (Knoxville, TN) and maintained at a 2 cm height of cut. After acclimating for 4 weeks, plants were treated with three rates of mesotrione (0.28, 0.35, and 0.42 kg ha-1), topramezone (0.018, 0.025, and 0.038 kg ha-1), and tembotrione (0.092, 0.184, and 0.276 kg ha-1) forming a 3 x 3 factorial, randomized complete block design with three replications. A non-treated control was also included for comparison. Percent visual bleaching (VB) and Fv/Fm data were collected 3, 7, 14, 21, 28, and 35 days after application. VB was rated using a 0 (dark green leaf tissue) to 100% (white leaf tissue) scale, while Fv/Fm data were collected using a hand-held pulse modulated fluorometer. Immediately following VB and Fv/Fm data collection, leaf material above 2 cm was harvested and frozen at -80°C. Chlorophyll and carotenoid pigments were extracted from harvested tissue and quantified via high-performance liquid chromatography. Chlorophyll, lutein, β-carotene, and xanthophyll cycle pigment concentrations were regressed over VB and Fv/Fm data. With the exception of zeaxanthin, both VB and Fv/Fm were linearly associated with all carotenoid and chlorophyll concentrations measured between 7 and 28 DAA; few significant relationships were detected at 3 and 35 DAA. R2 values never exceeded 0.65 on any date, suggesting neither evaluation method can accurately estimate carotenoid and chlorophyll pigment concentrations following HPPD inhibiting herbicide application." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Elmore, M. T., J. T. Brosnan, D. A. Kopsell, G. K. Breeden, and T. C. Mueller. 2011. Methods of assessing the activity of three HPPD inhibiting herbicides. South. Weed Sci. Soc. Proc. 64:p. 81. |
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| Web URL(s): http://www.swss.ws/wp-content/uploads/docs/2011 Proceedings-SWSS.pdf#page=189 Last checked: 08/05/2013 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: b2207931 |
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