| |
| Web URL(s): | http://www.jstor.org/stable/2557532
Last checked: 12/18/2015
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
|---|
| Publication Type:
| Refereed |
|---|
| Author(s): | Housley, T. L.;
Gibeaut, D. M.;
Carpita, N. C.;
Sims, I.;
Pollock, N. J. |
|---|
| Author Affiliation: | Departments of Agronomy and Botany & Plant Pathology, Purdue University; Department of Botany & Plant Pathology, Purdue University; Department of Botany & Plant Pathology, Purdue University; Environmental Biology Department, AFRC Institute for Grassland and Environmental Research, Welsh Plant Breeding Station, Plas Gogerddan; Environmental Biology Department, AFRC Institute for Grassland and Environmental Reasearch, Welsh Plant Breeding Station, Plas Gogerddan |
|---|
| Title: | Fructosyl transfer from sucrose and oligosaccharides during fructan synthesis in excised leaves of Lolium temulentum L. |
|---|
| Source: | New Phytologist. Vol. 119, No. 4, December 1991, p. 491-497. |
|---|
| Publishing Information: | Oxford, England: Cambridge University Press. |
|---|
| Keywords: | TIC Keywords: Fructans; Fructose; Sucrose; Carbohydrates; Leaves; Lolium temulentum
|
|---|
| Abstract/Contents: | Fructan biosynthesis besgins with the transfer of fructosyl moiety from one sucrose to another to yield a trisaccharide. Trisaccharides may also arise by the reversible transfer of a fructosyl moiety from higher oligomers to sucrose but in this case there is no net fructan synthesis. Short-term and long-term exposure of detached illuminated leaf blades of Lolium temulentum (L.) to 14CO2 was used to examine the mechanism of transfer of fructosyl residues to sucrose. Two trisaccharides, 1-kestose and neokestose, were found to be radioactive when leaves excised and illuminated for 15 h were exposed to 14CO2 for 30 min. The label increased in neokestose during the chase period, while that in 1-kestose increased for the first 2 h of the chase period then declined for the remaining 4 h. With a longer exposure to 14CO2 during the first 6 h of the induction period, three trisaccharides, neokestose, 1-kestose, and 6-kestose were radiolabelled. The label turned over in neokestose and 1-kestose, but continued to accumulate in 6-kestose during a subsequent 18 h chase period. The specific activities of glucose and fructose of the sucrosyl portion and the terminal fructosyl moiety of the various trisaccharides were compared. In the rapid pulse-chase experiment the specific activity of the terminal fructosyl moiety was consistently less than that of the sucrosyl moiety. During the chase period, the specific activity of the terminal and internal fructose moieties became similar. These results indicate that in addition to trisaccharide formed by transfer of fructosyl units from sucrose, substantial amounts of both neokestose and 1-kestose are made by transfer of fructosyl units form higher oligomers onto sucrose in reactions probalby localized in the vacuole. |
|---|
| Language: | English |
|---|
| References: | 26 |
|---|
| Note: | Pictures, b/w
Tables
Graphs |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Housley, T. L., D. M. Gibeaut, N. C. Carpita, I. Sims, and N. J. Pollock. 1991. Fructosyl transfer from sucrose and oligosaccharides during fructan synthesis in excised leaves of Lolium temulentum L.. New Phytol. 119(4):p. 491-497. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=22811 |
| If there are problems with this record, send us feedback about record 22811. |
| Choices for finding the above item: |
|---|
| Web URL(s):
http://www.jstor.org/stable/2557532
Last checked: 12/18/2015
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
| 
MSU catalog number: QK 1 .N38 |
| Find from within TIC:
Digitally in TIC by record number. |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
