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| Web URL(s): | http://www.jstor.org/stable/2557535
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| Publication Type:
| Refereed |
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| Author(s): | Smouter, Henk;
Simpson, Richard J. |
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| Author Affiliation: | School of Agriculture and Forestry, University of Melbourne |
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| Title: | Fructosyltransferase, invertase and fructan hydrolase activity |
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| Article Series: | Fructan metabolism in leaves of Lolium rigidum Gaudin, part 2 |
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| Source: | New Phytologist. Vol. 119, No. 4, December 1991, p. 517-526. |
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| Publishing Information: | Oxford, England: Cambridge University Press. |
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| # of Pages: | 10 |
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| Keywords: | TIC Keywords: Fructans; Metabolism; Leaves; Lolium rigidum; Hydrolases; Transferases; Invertase
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| Abstract/Contents: | This paper reports the conditions for the assay of sucrose-sucrose fructosyltransferase (SST); fructan-fructan fructosyltransferase (FFT), invertase and fructan hydrolase (FH) in extracts of leaves of Lolium rigidum Gaudin. Invertase had a temperature optimum between 30 and 35 C and a pH optimum between 4.5 and 5.0 whereas the enzymes of fructan metabolism had temperature optima at 25 C and pH optima at about 5.5. Analysis of the products of enzyme assays showed that crude SST activity produced mainly 1-kestose from 100mM sucrose and when incubated with 500mM sucrose, neokestose and possibly 6-kestose were also formed in low concentrations. FFT produced two tetrasaccharides and neokestose after incubation with 100 mM 1-kestose and FH produced fructose exclusively after incubation with high-molecular-weight fructan extracted from Lolium rigidum and Helianthus tuberosus L. and with a commercial preparation of bacterial levan. Enzyme activities were also measured during a phase of fructan accumulation. FH activity declined by 50%, but SST and FFT activities increased about 2.5-fold during this period. The data supported the proposed synthetic pathway of fructan accumulation from sucrose via 1-kestose into high-molecular-weight fructan and indicated that fructan synthesis may occur at low substrate concentrations. Extracts from leaves which had not accumulated fructans contained mesurable activities of SST and FFT. |
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| Language: | English |
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| References: | 25 |
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| See Also: | See also part 1 "Synthesis of fructan" New Phytologist, 119(4) December 1991, p. 509-516, R=28813. R=28813 |
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| Note: | Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Smouter, H., and R. J. Simpson. 1991. Fructosyltransferase, invertase and fructan hydrolase activity. New Phytol. 119(4):p. 517-526. |
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| Web URL(s):
http://www.jstor.org/stable/2557535
Last checked: 12/18/2015
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Access conditions: Item is within a limited-access website |
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MSU catalog number: QK 1 .N38 |
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