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| Web URL(s): | http://www.apsnet.org/publications/phytopathology/backissues/Documents/1992Articles/Phyto82n02_241.pdf#page=3
Last checked: 10/15/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Hayes, Chris;
Harman, Gary;
Woo, Sheri;
Gullino, Maria L. |
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| Author Affiliation: | Department of Horticultural Sciences, Cornell University |
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| Title: | Karotyping of Trichoderma Harzianum utilizing transverse alternating field electrophoresis (TAFE). |
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| Source: | Phytopathology. Vol. 82, No. 2, February 1992, p. 243-244. |
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| Publishing Information: | St. Paul, MN: American Phytopathological Society |
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| Keywords: | TIC Keywords: Chromosomes; Research; Protoplasts; Agar
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| Abstract/Contents: | "Recent techniques have been devised that allow the resolution if intact chromosomes. This new technology has been employed in an attempt to electrophoretically karyotype two strains of Ttrichoderma harzianum and a progeny formed by protoplast fusion of the two strains. Intact chromosomes were obtained by embedding viable protoplasts of parental strains, T12 and T95, and a fusion strain, 1295-22, into agar blocks. The protoplasts were lysed within the agar blocks, which greatly reduced shearing of the chromosomes. Three diverse run parameters were identified that allowed for visualization of chromosomes ranging in size from 100 Kilobase pairs (Kb) up to 6.0 Megabase pairs (Mb). The parental strains differed in the number and size of chromosomes. The fusion progeny had a karyotype that was similar to parental strain T12, with chromosomes of all strains ranging in size from 2.0 to 6.0 Mb." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Hayes, C., G. Harman, S. Woo, and M. L. Gullino. 1992. Karotyping of Trichoderma Harzianum utilizing transverse alternating field electrophoresis (TAFE).. Phytopathology. 82(2):p. 243-244. |
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| Web URL(s):
http://www.apsnet.org/publications/phytopathology/backissues/Documents/1992Articles/Phyto82n02_241.pdf#page=3
Last checked: 10/15/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: SB 599 .P48 |
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