| |
| Web URL(s): | http://www.apsnet.org/publications/phytopathology/backissues/Documents/1992Articles/Phyto82n02_241.pdf#page=7
Last checked: 10/15/2015
Requires: PDF Reader
Notes: Item is within a single large file |
|---|
| Publication Type:
| Report |
|---|
| Content Type: | Abstract or Summary only |
|---|
| Author(s): | Smith, Franzine;
Gadoury, David M.;
Harpending, Peter R.;
Sanford, John C. |
|---|
| Author Affiliation: | Departments of Horticultural Science and Plant Pathology, Cornell University |
|---|
| Title: | Transformation of a powdery mildew, uncinula necator, by microprojectile bombardment |
|---|
| Source: | Phytopathology. Vol. 82, No. 2, February 1992, p. 247. |
|---|
| Publishing Information: | St. Paul, MN: American Phytopathological Society |
|---|
| Keywords: | TIC Keywords: Seedlings; Aspergillus nidulans; Pathogens; Fungi
|
|---|
| Abstract/Contents: | "A benomyl-sensitive, single-spore clone of U. necator, growing on grapevine seedlings, was bombarded with tungsten particles coated with plasmid pbenA3; a pUC19 derivative that contains the benA3 allele of Aspergullus nidulans. This gene confers a high level of resistance to benomyl in Aspergillus. After bombardment, seedlings were sprayed every 7-10 days with 300 mg L-1 benomyl; which is lethal to the wild-type pathogen. After 3 weeks, conidia from colonies that continued to grow were transferred to benomyl-treated seedlings. Controls included (i) colonies that were not bombarded, (ii) colonies that were treated with naked particles, and (iii) seedlings that were not inoculated, but were sprayed with benomyl. Up to 10% of the colonies that were bombarded with pbenA3 became resistant to benomyl. Transformation was confirmed by isolation of DNA from the wild-type isolate and putative transformants, amplification of DNA with PCR using a primer sequence from the Aspergillus gene and comparision of DNA after restriction digestion. This is the first report of transformation of an obligately parasitic fungus." |
|---|
| Language: | English |
|---|
| References: | 0 |
|---|
| Note: | This item is an abstract only! |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Smith, F., D. M. Gadoury, P. R. Harpending, and J. C. Sanford. 1992. Transformation of a powdery mildew, uncinula necator, by microprojectile bombardment. Phytopathology. 82(2):p. 247. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=23069 |
| If there are problems with this record, send us feedback about record 23069. |
| Choices for finding the above item: |
|---|
| Web URL(s):
http://www.apsnet.org/publications/phytopathology/backissues/Documents/1992Articles/Phyto82n02_241.pdf#page=7
Last checked: 10/15/2015
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: SB 599 .P48 |
| Find from within TIC:
Digitally in TIC by record number. |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
