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| Publication Type:
| Refereed |
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| Author(s): | Dempsey, J. J.;
Wilson, I. D.;
Spencer-Phillips, P. T. N.;
Arnold, D. L. |
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| Title: | Assimilation of phosphite by Agrostis stolonifera L. and its in vitro effect on Microdochium nivale |
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| Meeting Info.: | Kristiansand, Norway: June 24-26, 2012 |
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| Source: | 3rd European Turfgrass Society Conference Proceedings. Vol. 3, 2012, p. Unknown. |
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| Publishing Information: | Angers, France: European Turfgrass Society |
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| # of Pages: | 0 |
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| Keywords: | TIC Keywords: Agrostis stolonifera; Cultivar evaluation; Disease development; Disease resistance; Microdochium nivale; Phosphite fungicides
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| Abstract/Contents: | "Phosphite (PO33-) has proven efficacy in reducing susceptibility to oomycete pathogens in many plant species, the possibility that phosphite can reduce susceptibility to Ascomycete pathogens such as Microdochium nivale in turfgrass is the subject of this research. This study aims to determine the infection processes and host responses in M. nivale challenged turfgrasses and whether phosphite treatments can increase resistance and thereby reduce susceptibility to infection. The processes involved in such increased resistance will be fully determined. This research is being carried out using a wide range of field trials and laboratory procedures. Results to date have produced significant and novel data. Field trials determined that PO33- treated plots of three species of cool-season turfgrasses, when compared with untreated controls, exhibited significantly lower percentages (p<0.01) of M. nivale infection during a period of high disease pressure. Analysis of Agrostis stolonifera tissues treated with a foliar application of PO33-, with High Performance Ion Chromatography (HPIC), provided data showing accumulations 48 h post application of 4889 ppm. The data confirmed full systemic mobility of PO33- and that no in planta conversion to PO43- occurred. In vitro studies using Potato Dextrose Agar (PDA), inoculated with M. nivale and amended with PO33- and PO43- ranging from 0.5 to 1000 g/ml, determined that PO33- concentrations of 100 g/ml and above, fully inhibited mycelial growth. Concentrations of 38 g/ml inhibited growth by 50%, compared to un-treated controls. PO43- amended PDA caused no inhibition. Confocal microscopy of PO33- treated tissues using the fluorescent indicator dyes dichlorofluorescein diacetate (H2DCFDA) and diaminofluorescein diacetate (DAF-2DA) is currently being used to determine if PO33- can increase synthesis of stress related compounds, hydrogen peroxide and nitric oxide. Further studies using HPIC and Gas Chromatography Mass Spectrometry will determine if PO33- treatment can increase synthesis of anti-microbial phenolic compounds and salicylic acid, thereby leading to an inducement of systemic resistance" |
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| Language: | English |
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| References: | Unknown |
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| Note: | Summary appears as abstract |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Dempsey, J. J., I. D. Wilson, P. T. N. Spencer-Phillips, and D. L. Arnold. 2012. Assimilation of phosphite by Agrostis stolonifera L. and its in vitro effect on Microdochium nivale. Eur. Turfgrass Soc. Conf. Proc. 3:p. Unknown. |
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