Full TGIF Record # 236285
Item 1 of 1
DOI:10.1007/s11032-013-9961-6
Web URL(s):https://link.springer.com/content/pdf/10.1007%2Fs11032-013-9961-6.pdf
    Last checked: 10/05/2017
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
https://link.springer.com/article/10.1007/s11032-013-9961-6/fulltext.html
    Last checked: 10/05/2017
    Access conditions: Item is within a limited-access website
Publication Type:
i
Refereed
Author(s):Wang, Junping; Pembleton, Luke W.; Baillie, Rebecca C.; Drayton, Michelle C.; Hand, Melanie L.; Bain, Melissa; Sawbridge, Timothy I.; Spangenberg, German C.; Forster, John W.; Cogan, Noel O. I.
Author Affiliation:Wang: Biosciences Research Division, Department of Environment and Primary Industries, Hamilton Centre, Hamilton; Pembleton, Baillie, Drayton, Hand, Bain, Sawbridge, Spangenberg, Forster, and Cogan: Biosciences Research Division, Department of Environment and Primary Industries; Wang, Pembleton, Baillie, Drayton, Hand, Bain, Sawbridge, Spangenberg, Forster, and Cogan: Dairy Futures Cooperative Research Centre, AgriBio, Centre for AgriBioscience; Pembleton, Sawbridge, Spangenberg, and Forster: La Trobe University, Bundoora, VIC, Australia
Title:Development and implementation of a multiplexed single nucleotide polymorphism genotyping tool for differentiation of ryegrass species and cultivars
Source:Molecular Breeding. Vol. 33, No. 2, February 2014, p. 435-451.
Publishing Information:The Netherlands: Kluwer Academic Publishers
# of Pages:17
Related Web URL:http://link.springer.com/article/10.1007/s11032-013-9961-6
    Last checked: 02/11/2014
    Notes: Abstract only
Keywords:TIC Keywords: Cultivar evaluation; Cultivar identification; Differentiation; Lolium multiflorum; Lolium perenne
Abstract/Contents:"Perennial ryegrass (Lolium perenne L.) and Italian ryegrass (Lolium multiflorum Lam.) are important temperate forage grasses which are closely related, generating fertile interspecific hybrids. All groups are represented by multiple cultivars in the commercial pasture seeds market. Due to the close taxonomic relationship between the two species, differentiation based on morphophysiological criteria is not always readily achievable. In addition, an obligate outbreeding reproductive habit produces high levels of individual heterozygosity and intrapopulation diversity, which presents problems for discrimination between cultivars. Molecular genetic marker polymorphism provides an effective means of addressing these challenges. An iterative process of resequencing from loci distributed across the perennial ryegrass genome was used to identify single nucleotide polymorphism (SNP) markers, which were then validated and formatted in a highly multiplexed (384-plex) assay system. SNP genotyping was then performed across samples of 48-192 individuals from a total of 27 ryegrass cultivars (19 of perennial ryegrass, seven of Italian ryegrass and one hybrid cultivar). SNP markers from perennial ryegrass exhibited a high level of transfer to Italian ryegrass. Data analysis permitted quantification of intra- and inter-species diversity, as well as discrimination between cultivars within each species, including diploid and autotetraploid cultivars of perennial ryegrass. Lower levels of SNP-based diversity were detected in Italian ryegrass than in perennial ryegrass. A neighbour-joining tree based on genetic distance analysis located a hybrid cultivar to an intermediate position between the two species-specific cultivar groups. The resulting catalogue of ryegrass cultivars will provide support for the processes of cultivar accreditation and quality assurance."
Language:English
References:51
Note:Figures
Tables
Graphs
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Wang, J., L. W. Pembleton, R. C. Baillie, J. C. Drayton, M. L. Hand, M. Bain, et al. 2014. Development and implementation of a multiplexed single nucleotide polymorphism genotyping tool for differentiation of ryegrass species and cultivars. Molecular Breeding. 33(2):p. 435-451.
Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=236285
If there are problems with this record, send us feedback about record 236285.
Choices for finding the above item:
DOI: 10.1007/s11032-013-9961-6
Web URL(s):
https://link.springer.com/content/pdf/10.1007%2Fs11032-013-9961-6.pdf
    Last checked: 10/05/2017
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
https://link.springer.com/article/10.1007/s11032-013-9961-6/fulltext.html
    Last checked: 10/05/2017
    Access conditions: Item is within a limited-access website
Find from within TIC:
   Digitally in TIC by record number.
Request through your local library's inter-library loan service (bring or send a copy of this TGIF record)