| |
Publication Type:
| Report |
Author(s): | Feng, Xia;
Sun, Zhen-yuan;
Han, Lei;
Peng, Zhen-hua |
Author Affiliation: | The Laboratory of Biotechnology, Chinese Academy of Forestry, Beijing, China |
Title: | Callus induction and plant regeneration of perennial ryegrass |
Source: | [Caoye Kexue] [Pratacultural Science]. Vol. 21, No. 10, October 15 2004, p. 23-28. |
Publishing Information: | [Lanzhou Shi, China]: ["Cao Ye Ke Xue" Bian Jibu] |
# of Pages: | 6 |
Abstract/Contents: | "The objective of this study was to optimize tissue culture responses of turf-type perennial ryegrass (Lolium perenne L.) for genetic transformation, the effects of medium supplements on tissue culture responses were investigated with mature seeds of an elite cultivar, 'Derby', as explant. The best callus induction was obtained in N6 medium with 10 mg/L 2,4-D, 500 mg/L CH, 0.5 mg/L 6-BA and 0.5 mg/L NAA, of which the induction rate was 85%. The highest differentiation rate, 77.13%, was achieved in the medium contained N6 medium and 1 mg/L 6-BA, 0.5 mg/L KT. The root inducing medium, containing 0.2 mg/L NAA, 30 g/L Sucrose and N6 medium with half macro, gave 100% root inducing rate." |
Language: | Chinese |
References: | 15 |
Note: | Abstract also appears in English Pictures, b/w Tables |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Feng, X., Z.-y. Sun, L. Han, and Z.-h. Peng. 2004. Callus induction and plant regeneration of perennial ryegrass. (In Chinese) [Caoye Kexue] [Pratacultural Science]. 21(10):p. 23-28. |
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