| |
| Web URL(s): | http://cykx.lzu.edu.cn//EN/abstract/abstract9642.shtml
Last checked: 03/06/2014
Notes: Guide page with English abstract
http://cykx.lzu.edu.cn//EN/article/downloadArticleFile.do?attachType=PDF&id=9642
Last checked: 03/06/2014
Requires: PDF Reader |
|---|
| Publication Type:
| Report |
|---|
| Author(s): | Ran, Xiao-wei;
Wang, Yu;
Yuan, Qing-hua |
|---|
| Author Affiliation: | Ren: College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou; Ren, Wang, and Yuan: Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing, China |
|---|
| Title: | Optimization of SRAP-PCR system on Poa pratensis using orthogonal design and selection of primers |
|---|
| Source: | [Caoye Kexue] [Pratacultural Science]. Vol. 29, No. 3, March 15 2012, p. 411-416. |
|---|
| Publishing Information: | [Lanzhou Shi, China]: ["Cao Ye Ke Xue" Bian Jibu] |
|---|
| # of Pages: | 6 |
|---|
| Abstract/Contents: | "An orthogonal design was used to optimize a SRAP-PCR system for Poa pratensis with 4 factors (Mg2+, dNTP, primer and Taq polymerase) at 3 levels plus the concentration of template DNA. The optimized SRAP-PCR system was: 2 μL 10 x PCR buffer, 40 ng template DNA, Mg2+1.75 mmol·L-1, dNTP 220 μmol·L-1, primer 0.25 μmol·L-1, Taq DNA polymerase 1.0 U in a total of 20 μL reaction mixtures. With the optimized system, 43 primer combinations were selected among 100 primer combinations, which produced abundant polymorphism bands. This study optimized SRAP-PCR system and selected the proper primers in P. pratensis, which would play an important role in genetic diversity analyses, map construction, germplasm identification in P. pratensis with SRAP markers." |
|---|
| Language: | Chinese |
|---|
| References: | 27 |
|---|
| Note: | Abstract also appears in English
"Sum No. 224"
Pictures, color & b/w
Tables |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Ran, X.-w., Y. Wang, and Q.-h. Yuan. 2012. Optimization of SRAP-PCR system on Poa pratensis using orthogonal design and selection of primers. (In Chinese) [Caoye Kexue] [Pratacultural Science]. 29(3):p. 411-416. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=237372 |
| If there are problems with this record, send us feedback about record 237372. |
| Choices for finding the above item: |
|---|
| Web URL(s):
http://cykx.lzu.edu.cn//EN/abstract/abstract9642.shtml
Last checked: 03/06/2014
Notes: Guide page with English abstract
http://cykx.lzu.edu.cn//EN/article/downloadArticleFile.do?attachType=PDF&id=9642
Last checked: 03/06/2014
Requires: PDF Reader |
| 
MSU catalog number: b10275836 |
| Find from within TIC:
Digitally in TIC by record number. |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
