Full TGIF Record # 237479
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Author(s):Hu, Zhang-hua; Chen, Huo-qing; Wu, Guan-ting; Zhang, Xi-liang; Jin, Wei; Xia, Zheng-hong; Chen, Jin-qing
Author Affiliation:Hu, Wu, Jin, and Chen, J.-q.: Institute for Application of Atomic Energy, Zhejiang Academy of Agricultural Sciences, Hangzhou; Chen, H.-q., Zhang, and Xia: Zhejiang Oasis Agriculture Co. Ltd, Shaoxing, China
Title:Establishment of suspension cell lines and high frequency regeneration of green plants in tall fescue
Source:[Caoye Xuebao] [Acta Prataculturae Sinica]. Vol. 12, No. 3, June 20 2003, p. 95-99.
# of Pages:5
Publishing Information:[Lanzhou Shi, China]: ["Cao Ye Xue Bao" Bian Ji Wei Yuan Hui]
Abstract/Contents:"Sterilized mature seeds of five tall fescue (Festuca rubra) varieties were cultured for callus induction on N6 medium containing different concentrations of 2,4-D and ABA. The results showed that there were significant influences of different concentrations of 2,4-D and ABA on callus induction frequency of these varieties, with the highest induction frequency for supplement of 9 mg/L 2,4-D and 2 mg/L ABA in the medium. In addition, marked differences in induction frequency were also observed among the five varieties. High quality embryogenic callus resulting from several times subcultures were transferred to AA [ABA] medium for suspension culture. Eight suspension cell lines of three varieties were finally established. After high osmosis treatment, green plants were regenerated with high frequency from six lines, largely overcoming the problem of severe albino of regeneration plants in previous cell suspension cultures."
Note:Abstract also appears in English
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Hu, Z.-h., H.-q. Chen, G.-t. Wu, X.-l. Zhang, W. Jin, Z.-h. Xia, et al. 2003. Establishment of suspension cell lines and high frequency regeneration of green plants in tall fescue. (In Chinese) [Caoye Xuebao] [Acta Prataculturae Sinica]. 12(3):p. 95-99.
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