Full TGIF Record # 237491
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Publication Type:
i
Report
Author(s):Zhang, Lei; Wu, Dian-xing; Hu, Fan-rong; Wang, Hai-qiu; Ma, Chuan-xi
Author Affiliation:Zhang, Wu, Hu, and Wang: Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou; Zhang and Ma: Agronomy Department, Anhui Agricultural University, Hefei, China
Title:Optimization of major factors for tissue culture and Agrobacterium-mediated genetic transformation of Japanese lawngrass (Zoysia japonica)
Source:[Caoye Xuebao] [Acta Prataculturae Sinica]. Vol. 13, No. 4, August 20 2004, p. 100-105.
# of Pages:6
Publishing Information:[Lanzhou Shi, China]: ["Cao Ye Xue Bao" Bian Ji Wei Yuan Hui]
Abstract/Contents:"The major factors for callus induction, callus differentiation, and Agrobacterium-mediated transformation were studied in the current paper, using dormancy-broken seeds as the experiment material. The results indicated that the optimal culture medium for callus induction is MS containing 2,4-D 1 mg/L and NAA 3 mg/L, for callus differentiation is MS containing 6-BA 3 mg/L and KT 3.5 mg/L, and for root formation is MS containing 0.3 mg/L NAA. The GUS transient expression study indicated the optimised factors for genetic transformation were as follows: Use 2-week-recultured calluses as the original material, infect for 10 minutes in Agrobacterium solution under negative pressure treatment, then follow with mutual culture under continuous light."
Language:Chinese
References:19
Note:Abstract also appears in English
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Zhang, L., D.-x. Wu, F.-r. Hu, H.-q. Wang, and C.-x. Ma. 2004. Optimization of major factors for tissue culture and Agrobacterium-mediated genetic transformation of Japanese lawngrass (Zoysia japonica). (In Chinese) [Caoye Xuebao] [Acta Prataculturae Sinica]. 13(4):p. 100-105.
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