| |
| Publication Type:
| Report |
|---|
| Author(s): | Zhang, Lei;
Wu, Dian-xing;
Hu, Fan-rong;
Wang, Hai-qiu;
Ma, Chuan-xi |
|---|
| Author Affiliation: | Zhang, Wu, Hu, and Wang: Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou; Zhang and Ma: Agronomy Department, Anhui Agricultural University, Hefei, China |
|---|
| Title: | Optimization of major factors for tissue culture and Agrobacterium-mediated genetic transformation of Japanese lawngrass (Zoysia japonica) |
|---|
| Source: | [Caoye Xuebao] [Acta Prataculturae Sinica]. Vol. 13, No. 4, August 20 2004, p. 100-105. |
|---|
| Publishing Information: | [Lanzhou Shi, China]: ["Cao Ye Xue Bao" Bian Ji Wei Yuan Hui] |
|---|
| # of Pages: | 6 |
|---|
| Abstract/Contents: | "The major factors for callus induction, callus differentiation, and Agrobacterium-mediated transformation were studied in the current paper, using dormancy-broken seeds as the experiment material. The results indicated that the optimal culture medium for callus induction is MS containing 2,4-D 1 mg/L and NAA 3 mg/L, for callus differentiation is MS containing 6-BA 3 mg/L and KT 3.5 mg/L, and for root formation is MS containing 0.3 mg/L NAA. The GUS transient expression study indicated the optimised factors for genetic transformation were as follows: Use 2-week-recultured calluses as the original material, infect for 10 minutes in Agrobacterium solution under negative pressure treatment, then follow with mutual culture under continuous light." |
|---|
| Language: | Chinese |
|---|
| References: | 19 |
|---|
| Note: | Abstract also appears in English
Graphs |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Zhang, L., D.-x. Wu, F.-r. Hu, H.-q. Wang, and C.-x. Ma. 2004. Optimization of major factors for tissue culture and Agrobacterium-mediated genetic transformation of Japanese lawngrass (Zoysia japonica). (In Chinese) [Caoye Xuebao] [Acta Prataculturae Sinica]. 13(4):p. 100-105. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=237491 |
| If there are problems with this record, send us feedback about record 237491. |
| Choices for finding the above item: |
|---|
| 
MSU catalog number: b10289426 |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
