Full TGIF Record # 237497
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Publication Type:
i
Report
Author(s):Yang, Ai-fang; He, Chun-mei; Wang, Xian-li; Zhang, Ju-ren
Author Affiliation:School of Life Science, Shangdong University, Jinan, China
Title:In vitro culture and plant regeneration from immature ears of Lolium perenne and Lolium multiflorum varieties
Source:[Caoye Xuebao] [Acta Prataculturae Sinica]. Vol. 13, No. 5, October 20 2004, p. 84-90.
# of Pages:7
Publishing Information:[Lanzhou Shi, China]: ["Cao Ye Xue Bao" Bian Ji Wei Yuan Hui]
Abstract/Contents:"Calli were induced from segments of immature ears of Lolium perenne and Lolium multiflorum varieties respectively on modified MS media supplemented with appropriate concentration of 2,4-D, usually 1 mg/L. The frequency of calli induction could reach 95%. Some calli developed adventitious buds directly on the induction media. Some calli with higher ability of differentiation produced large amounts of buds or shoots after being cultured on the differentiation media. The factors influenced the frequency of calli induction of 6-BA and 2,4-D in the media played important roles on the calli subculture and plant regeneration as well as the genotypes of ryegrass affected the calli induction and plant regeneration. This system, with advantages of short-period, less genotype-dependence and higher plantlet regeneration, was available for cell engineering and genetic manipulation for ryegrass."
Language:Chinese
References:16
Note:Abstract also appears in English
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Yang, A.-f., C.-m. He, X.-l. Wang, and J.-r. Zhang. 2004. In vitro culture and plant regeneration from immature ears of Lolium perenne and Lolium multiflorum varieties. (In Chinese) [Caoye Xuebao] [Acta Prataculturae Sinica]. 13(5):p. 84-90.
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