Full TGIF Record # 237531
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Publication Type:
i
Report
Author(s):Jia, Wei-long; Yang, Li-li; Zhang, Yan-qin; Hu, Yuan-lei; Ni, Ting; Wu, Qi; Lin, Zhong-pin
Author Affiliation:Jia: School of Sciences, Tianjin University, Tianjin; Yang and Zhang: Arid Farming Research Center, Shanxi Academy of Agricultural Sciences, Taiyuan; Hu, Ni, Wu, and Lin: College of Life Sciences, Peking University, Beijing, China
Title:Establishment of high frequency plantlet regeneration system of Festuca rubra and GUS gene transformation
Source:[Caoye Xuebao] [Acta Prataculturae Sinica]. Vol. 14, No. 4, August 20 2005, p. 53-57.
# of Pages:5
Publishing Information:[Lanzhou Shi, China]: ["Cao Ye Xue Bao" Bian Ji Wei Yuan Hui]
Abstract/Contents:"Mature seeds of Festuca rubra were used for explants. Effects of 2,4-D, organic material, and illumination, on callus induction, growth state and shoot regeneration were studied. Results indicated that a 2,4-D concentration of 3-5 mg/L was optimal for callus induction and callus subculture. The frequency of callus induction on the medium supplemented with 3-5 mg/L of 2,4-D ranged from 46.6%-57.9% with the callus keeping an embryogenic state during subculture. The rate of plant regeneration from callus which were subcultured more than two years was approximately 99% in the medium when supplemented with 3-5 mg/L 2,4-D + 500 mg/L proline + 130 mg/L L-Asparginem and used in conjunction with 15 days illumination every one to two months during callus darkness subculture. By using the callus which was subcultured more than two years, transient expression of the GUS gene was observed on the callus after particle bombardment."
Language:Chinese
References:10
Note:Abstract also appears in English
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Jia, W.-l., L.-l. Yang, Y.-q. Zhang, Y.-l. Hu, T. Ni, Q. Wu, et al. 2005. Establishment of high frequency plantlet regeneration system of Festuca rubra and GUS gene transformation. (In Chinese) [Caoye Xuebao] [Acta Prataculturae Sinica]. 14(4):p. 53-57.
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