Full TGIF Record # 237545
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Author(s):Wang, Wei-xia; Hu, Zhang-hua; Chen, Jin-qing; Xuan, Song-nan
Author Affiliation:Wang and Xuan: China National Rice Research Institute, Hangzhou; Hu and Chen: Zhejiang Academy of Agricultural Sciences, Hangzhou, China
Title:Embryogenic callus induction and plant regeneration from mature seed of Zoysia japonica cv. Songnan
Source:[Caoye Xuebao] [Acta Prataculturae Sinica]. Vol. 15, No. 3, June 20 2006, p. 132-137.
# of Pages:6
Publishing Information:[Lanzhou Shi, China]: ["Cao Ye Xue Bao" Bian Ji Wei Yuan Hui]
Abstract/Contents:"Callus induction was induced by inoculating sterilized, mature Zoysia japonica cv. Songnan seeds with broken dormancy on solidified MS and N6 media containing different concentrations of 2,4-D. The results showed that the main reason for dormancy of Z. japonica seed was the hull. There were no significant effects of concentrations of 2,4-D between 2-5 mg/L or of basal medium MS and N6 on callus induction frequency and appearance. The appearance of primary callus was white, translucent, and sticky and was accompanied by a high water content. Subculturing onto a medium supplemented with mannitol, maltose, or ABA and with prolonged subculture time promoted embryogenic callus induction. For embryogenic callus induction and maintenance, basal medium MS was better than N6. Shoots were regenerated from embryogenic callus in MS medium containing 0.1 mg/L 2,4-D."
Note:Abstract also appears in English
Pictures, b/w
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Wang, W.-x., Z.-h. Hu, J.-q. Chen, and S.-n. Xuan. 2006. Embryogenic callus induction and plant regeneration from mature seed of Zoysia japonica cv. Songnan. (In Chinese) [Caoye Xuebao] [Acta Prataculturae Sinica]. 15(3):p. 132-137.
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