Full TGIF Record # 237592
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Author(s):Wang, Zhi-yong; Yuan, Xue-jun; Liu, Jian-xiu; Guo, Hai-lin
Author Affiliation:Wang: Department of Horticulture, Nanjing Agricultural University [University]; Wang, Yuan, Liu, Guo: Instutute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, China
Title:Optimization for SRAP - PCR system in Cynodon dactylon and selection of primers
Source:[Caoye Xuebao] [Acta Prataculturae Sinica]. Vol. 17, No. 3, June 20 2008, p. 79-85.
# of Pages:7
Publishing Information:[Lanzhou Shi, China]: ["Cao Ye Xue Bao" Bian Ji Wei Yuan Hui]
Abstract/Contents:"Cynodon dactylon genomic DNA from leaf tissue was isolated according to the SDS proceduce [procedure]. An orthogonal diagram as experimental design was applied to study the effects of three elvels of Mg2+, dNTP, primers and Taq DNA polymerase respectively on the reaction system of SRAP, and the SRAP reaction system was finally optimized by analyzed effects of the concentrations of DNA template. The results showed that all factors had the significant effect on the results of SRAP-PCR. And a better amplification of SRAP-PCA was obtained with the reaction system for C. dactylon containing 2 μL 10Xbuffer, 40 ng genomic templates DNA, 1.25 mmol/L Mg2+, 260 μmol/L dNTA, 0.2 μmol/L Primer, 1.0 U Taq DNA polymerase in the total volune if 29 μL. At the same time, 34 primer combinations were selected with the optimized system among 90 primer combinations, which had abundant polymorphism bands. This study would be conducted to the optimized SRAP-PCR system and selection of primers which would play an important role in C. dactylon genetic diversity analysis, map construction, gene localization of important traits, germplasm identification in C. dactylon with SRAP markers"
Note:Abstract also appears in English
"Sum No. 74"
Pictures, b/w
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Wang, Z.-y., X.-j. Yuan, J.-x. Liu, and H.-l. Guo. 2008. Optimization for SRAP - PCR system in Cynodon dactylon and selection of primers. (In Chinese) [Caoye Xuebao] [Acta Prataculturae Sinica]. 17(3):p. 79-85.
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