Full TGIF Record # 237600
Item 1 of 1
DOI:10.11686/cyxb20080614
Web URL(s):http://cyxb.lzu.edu.cn/CN/article/downloadArticleFile.do?attachType=PDF&id=572
    Last checked: 02/11/2016
    Requires: PDF Reader
Publication Type:
i
Report
Author(s):Xue, Dan-dan; Zheng, Yi-qi; Wang, Zhi-yong; Guo, Hai-lin; Chen, Xuan; Liu, Jian-xiu
Author Affiliation:Xue: College of Horticulture, Nanjing Agricultural University; Xue, Zheng, Wang, Guo, Chen, and Liu: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, China
Title:Establishment and optimization of SRAP-PCR reaction system for Zoysia
Source:[Caoye Xuebao] [Acta Prataculturae Sinica]. Vol. 17, No. 6, December 20 2008, p. 93-101.
# of Pages:9
Publishing Information:[Lanzhou Shi, China]: ["Cao Ye Xue Bao" Bian Ji Wei Yuan Hui]
Abstract/Contents:"Genetic DNA of Zosia extracted by the SDS method was used as a template. The major factors of SRAP, such as concentrations of Mg2+, dNTPs, primers, Taq DNA polymerase, and DNA template, were optimized in this study by single factor tests and orthogonal design of five factors at four levels. In single factor tests, the impacts of the SRAP-PCR system were studied for each factor at various levels. In the orthogonal design test, the components of SRAP were considered comprehensively, and a suitable level of each factor was obtained by the intuitional analysis method. Comparing results of four primers and six materials validation, a few differences were found in the amplified electrophoresis of two reaction systems. A comprehensive and deep analysis of the bands, polymorphic bands and polymorphism rate in the two amplified results resulted in an optimised SRAP-PCR system for Zoysia being established: MG2+ 2.0 mmol/L, dNTP 220 μmol/L, primer 0.2 μmol/L, Taq DNA polymerase 0.5 U, DNA template 60 ng, 10 × buffer 2μL in the 20 μL volume reaction. The optimized SRAP-PCR reaction system showed that SRAP markers could be widely used for genetic diversity, germplasm identification, construction of genetic linkage maps and relative analysis in Zoysia."
Language:Chinese
References:29
Note:Abstract also appears in English
"Sum No. 77"
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Xue, D.-d., Y.-q. Zheng, Z.-y. Wang, H.-l. Guo, X. Chen, and J.-x. Liu. 2008. Establishment and optimization of SRAP-PCR reaction system for Zoysia. (In Chinese) [Caoye Xuebao] [Acta Prataculturae Sinica]. 17(6):p. 93-101.
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DOI: 10.11686/cyxb20080614
Web URL(s):
http://cyxb.lzu.edu.cn/CN/article/downloadArticleFile.do?attachType=PDF&id=572
    Last checked: 02/11/2016
    Requires: PDF Reader
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