Full TGIF Record # 237667
Item 1 of 1
DOI:10.11686/cyxb20100622
Web URL(s):http://cyxb.lzu.edu.cn/EN/article/downloadArticleFile.do?attachType=PDF&id=2826
    Last checked: 02/11/2016
    Requires: PDF Reader
Publication Type:
i
Report
Author(s):Wang, Zhou; Zong, Jun-qin; Xuan, Ji-ping; Guo, Ai-gui; Liu, Jian-xiu
Author Affiliation:Wang: College of Life Sciences, Nanjing Agricultural University; Wang, Zong, Xuan, Guo, and Liu: Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing, China
Title:Cloning and sequence analysis of full-length cDNA of an actin gene from zoysia grass (Zoysia japonica)
Source:[Caoye Xuebao] [Acta Prataculturae Sinica]. Vol. 19, No. 6, December 20 2010, p. 154-163.
# of Pages:10
Publishing Information:[Lanzhou Shi, China]: ["Cao Ye Xue Bao" Bian Ji Wei Yuan Hui]
Abstract/Contents:"One 1560 bp full-length cDNA of Actin was cloned from leaves of Zoysia grass using RT-PCR and RACE methods. The sequence analysis revealed that it contained an ORF of 1134 bp encoding a protein composed of 377 amino acids, a 5'-UTR of 157 bp and a 3'-UTR of 495 bp. Homologous alignment showed that it shared over 85% of nucleotide identities and 97% of amino acid identities with actins from other plants in Gen-Bank. The full-length cDNA was designated as ZjACT with its accession number GU290545. The phylogenetic tree constructed on the basis of amino acid sequences suggested that the relationship of actins from zoysia grass was most intimate with that from barley and poulard wheat, and they might share the same differential time in evolution. A comparison of the cDNA and its genomic counterpart (GU290546) demonstrated that the gene consisted of 4 exons and 3 introns. These results provided clues for evolutionary studies of the actin gene family, and on its functional and evolutionary diversity, as well as served as references for functional analysis and utilization of Actin gene from turf and forage grasses."
Language:Chinese
References:41
Note:Abstract also appears in English
"Sum No. 89"
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Wang, Z., J.-q. Zong, J.-p. Xuan, A.-g. Guo, and J.-x. Liu. 2010. Cloning and sequence analysis of full-length cDNA of an actin gene from zoysia grass (Zoysia japonica). (In Chinese) [Caoye Xuebao] [Acta Prataculturae Sinica]. 19(6):p. 154-163.
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DOI: 10.11686/cyxb20100622
Web URL(s):
http://cyxb.lzu.edu.cn/EN/article/downloadArticleFile.do?attachType=PDF&id=2826
    Last checked: 02/11/2016
    Requires: PDF Reader
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