Full TGIF Record # 237717
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Web URL(s):http://cyxb.lzu.edu.cn/EN/article/downloadArticleFile.do?attachType=PDF&id=3062
    Last checked: 02/11/2016
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Author(s):Zhao, Xiao-qiang; Ma, Hui-ling; Lin, Dong; Zhou, Wan-hai; Wu, Xiang
Author Affiliation:College of Pratacultural Science, Gansu Agricultural University and Key Laboratory of Grassland Ecosystem, Ministry of Education and Sino-U.S. Centers for Grazingland Ecosystem Sustainability, Lanzhou, China
Title:Culture and plant regeneration of protoplasts from embryogenic calli of Nuglade (Poa pratensis)
Source:[Caoye Xuebao] [Acta Prataculturae Sinica]. Vol. 19, No. 2, April 20 2010, p. 55-60.
# of Pages:6
Publishing Information:[Lanzhou Shi, China]: ["Cao Ye Xue Bao" Bian Ji Wei Yuan Hui]
Abstract/Contents:"Embryogenic calli were induced from mature seeds of Nuglade (Poa Pratensis) on MB5 medium supplemented with 3.0 mg/L 2,4-D, 0.5 mg/L 6-BA. Protoplasts were isolated from the embryogeni calli after subculture for 7-9 months. The protoplasts were cultured on KM8 P medium supplemented with 3.0 mg/L 2,4-D, 0.5 mg/L 6-BA, 100 mg/L casein hydrolysate, 100 mg/L lactablumin hydrolysate, 1% (W/V) sucrose and 0.4 mol/L mannitol. The first divisions happened after 3 days and small cell clusters appeared after 2 to 3 weeks in the culture medium. The protoplast-derived cells could keep dividing sustainably and forming calli by adding fresh protoplast culture liquids once or twice to lower osmotic pressure. The regenerated calli, 3 to 5 mm in diameter, were transferred to solid medium (MS medium supplements with 3.0 mg/L 2,4-D, 0.5 mg/L 6-BA then MS medium supplements with 0.5 mg/L NAA, 5.0 mg/L 6-BA) to carry out subculture for cell propagation, differentiation, then formation of complete plants."
Note:Abstract also appears in English
"Sum No. 85"
Pictures, color
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Zhao, X.-q., H.-l. Ma, D. Lin, W.-h. Zhou, and X. Wu. 2010. Culture and plant regeneration of protoplasts from embryogenic calli of Nuglade (Poa pratensis). (In Chinese) [Caoye Xuebao] [Acta Prataculturae Sinica]. 19(2):p. 55-60.
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DOI: 10.11686/cyxb20100208
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    Last checked: 02/11/2016
    Requires: PDF Reader
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MSU catalog number: b10289426
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