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| DOI: | 10.11686/cyxb20100208 |
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| Web URL(s): | http://cyxb.lzu.edu.cn/EN/article/downloadArticleFile.do?attachType=PDF&id=3062
Last checked: 02/11/2016
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Author(s): | Zhao, Xiao-qiang;
Ma, Hui-ling;
Lin, Dong;
Zhou, Wan-hai;
Wu, Xiang |
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| Author Affiliation: | College of Pratacultural Science, Gansu Agricultural University and Key Laboratory of Grassland Ecosystem, Ministry of Education and Sino-U.S. Centers for Grazingland Ecosystem Sustainability, Lanzhou, China |
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| Title: | Culture and plant regeneration of protoplasts from embryogenic calli of Nuglade (Poa pratensis) |
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| Source: | [Caoye Xuebao] [Acta Prataculturae Sinica]. Vol. 19, No. 2, April 20 2010, p. 55-60. |
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| Publishing Information: | [Lanzhou Shi, China]: ["Cao Ye Xue Bao" Bian Ji Wei Yuan Hui] |
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| # of Pages: | 6 |
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| Abstract/Contents: | "Embryogenic calli were induced from mature seeds of Nuglade (Poa Pratensis) on MB5 medium supplemented with 3.0 mg/L 2,4-D, 0.5 mg/L 6-BA. Protoplasts were isolated from the embryogeni calli after subculture for 7-9 months. The protoplasts were cultured on KM8 P medium supplemented with 3.0 mg/L 2,4-D, 0.5 mg/L 6-BA, 100 mg/L casein hydrolysate, 100 mg/L lactablumin hydrolysate, 1% (W/V) sucrose and 0.4 mol/L mannitol. The first divisions happened after 3 days and small cell clusters appeared after 2 to 3 weeks in the culture medium. The protoplast-derived cells could keep dividing sustainably and forming calli by adding fresh protoplast culture liquids once or twice to lower osmotic pressure. The regenerated calli, 3 to 5 mm in diameter, were transferred to solid medium (MS medium supplements with 3.0 mg/L 2,4-D, 0.5 mg/L 6-BA then MS medium supplements with 0.5 mg/L NAA, 5.0 mg/L 6-BA) to carry out subculture for cell propagation, differentiation, then formation of complete plants." |
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| Language: | Chinese |
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| References: | 18 |
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| Note: | Abstract also appears in English
"Sum No. 85"
Pictures, color
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Zhao, X.-q., H.-l. Ma, D. Lin, W.-h. Zhou, and X. Wu. 2010. Culture and plant regeneration of protoplasts from embryogenic calli of Nuglade (Poa pratensis). (In Chinese) [Caoye Xuebao] [Acta Prataculturae Sinica]. 19(2):p. 55-60. |
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| DOI: 10.11686/cyxb20100208 |
| Web URL(s):
http://cyxb.lzu.edu.cn/EN/article/downloadArticleFile.do?attachType=PDF&id=3062
Last checked: 02/11/2016
Requires: PDF Reader |
| 
MSU catalog number: b10289426 |
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