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DOI:10.11686/cyxb20120218
Web URL(s):http://en.cnki.com.cn/Article_en/CJFDTOTAL-CYXB201202017.htm
    Last checked: 02/10/2016
Publication Type:
i
Report
Author(s):An, Hui-hui; Ma, Hui-ling; Li, Jian; Bai, Sheng-jun; Ma, Xiang
Author Affiliation:An, Ma, H.-l., Bai, and Ma, X.: Key Laboratory of Grassland Ecosystem, Gansu Agricultural University and Sino-U.S. Centers for Grazingland Ecosystem Sustainability, Lanzhou; An and Ma, H.-l.: Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Lanzhou; Li: Key Laboratory of Water Cycle and Related Land Surface Processes, Institute of Geographic Sciences and Natural Resources Research, CAS, Beijing, China
Title:A study on transformation and expression of the Lyz-GFP genes mediated by agrobacteria in creeping bentgrass variety Penn A-1
Source:[Caoye Xuebao] [Acta Prataculturae Sinica]. Vol. 21, No. 2, April 20 2012, p. 141-148.
# of Pages:8
Publishing Information:[Lanzhou Shi, China]: ["Cao Ye Xue Bao" Bian Ji Wei Yuan Hui]
Abstract/Contents:"Mature embryos of bentgrass (Agrostis stolonifera) Penn A-1 were used to establish a highly efficient regeneration system. The dual genes Lyz-GFP (Lysozyme gene and green fluorescence protein gene) were transformed into embryonic calli of creeping bentgrass by an agrobacterium-mediation method to obtain transgenic plants with disease resistance capability. The best conditions for transformation of the Lyz-GFP genes in Penn A-1 were studied. The callus induction ratio of Penn A-1 was highest at 36% on MS medium supplemented with 2.0 mg/L 2,4-D and 0.1 mg/L 6-BA, and the calli had the optimum growth status. The highest differentiation ratio was 42.5% on MSO medium supplemented with 0.5 mg/L NAA. The study also showed that growth of agrobacterium LBA4404 was restrained by 300 mg/L cefotaxime (Cef). Transformed calli performed well in later growth periods as well as in the regeneration of transgenic plants after infection with agrobacterium LBA4404 (OD600: 0.3-0.5) carried with pBI121-Lyz-GFP for 10-15 min, and co-cultured for 3 days. The transformation ratio of calli was 12.5%; the regeneration ratio of transgenic plants was 27.5%; the transgenic plants strongly expressed fluorescence, and the 750 bp target fragments of the GFP gene were amplified by PCR from 2 acquired transgenic plants with expression of Penn A-1 fluorescence."
Language:Chinese
References:29
Note:Abstract also appears in English
"Sum No. 97"
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
An, H.-h., H.-l. Ma, J. Li, S.-j. Bai, and X. Ma. 2012. A study on transformation and expression of the Lyz-GFP genes mediated by agrobacteria in creeping bentgrass variety Penn A-1. (In Chinese) [Caoye Xuebao] [Acta Prataculturae Sinica]. 21(2):p. 141-148.
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DOI: 10.11686/cyxb20120218
Web URL(s):
http://en.cnki.com.cn/Article_en/CJFDTOTAL-CYXB201202017.htm
    Last checked: 02/10/2016
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