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| DOI: | 10.1016/j.funbio.2014.01.003 |
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| Web URL(s): | http://www.sciencedirect.com/science/article/pii/S1878614614000105
Last checked: 03/25/2014
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Zhou, Yanfei;
Bradshaw, Rosie E.;
Johnson, Richard D.;
Hume, David E.;
Simpson, Wayne R.;
Schmid, Jan |
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| Author Affiliation: | Zhou, Bradshaw, and Schmid: Institute of Fundamental Sciences, College of Sciences, Massey University; Johnson, Hume, and Simpson: AgResearch Grasslands Research Center, Palmerston North, New Zealand |
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| Title: | Detection and quantification of three distinct Neotyphodium lolii endophytes in Lolium perenne by real time PCR of secondary metabolite genes |
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| Source: | Fungal Biology. Vol. 118, No. 3, March 2014, p. 316-324. |
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| Publishing Information: | [Amsterdam]: Elsevier |
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| # of Pages: | 9 |
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| Keywords: | TIC Keywords: Endophytes; Identification; Lolium perenne; Neotyphodium lolii; Polymerase chain reaction; Toxicity
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| Abstract/Contents: | "Perennial ryegrass (Lolium perenne) is a widely used pasture grass, which is frequently infected by Neotyphodium lolii endophytes that enhance grass performance but can produce alkaloids inducing toxicosis in livestock. Several selected endophyte strains with reduced livestock toxicity, but that confer insect resistance, are now in common use. Little is known regarding the survival and persistence of these endophytes when in competition with common toxic endophytes. This is mainly because there are currently no assays available to easily and reliably quantify different endophytes in pastures or in batches of seeds infected with multiple strains. We developed real time PCR assays, based on secondary metabolite genes known to differ between N. lolii endophyte strains, to quantify two selected endophytes, AR1 and AR37, and a common toxic ecotype used in New Zealand. A duplex PCR allowed assessment of endophyte:grass DNA ratios with high sensitivity, specificity and precision. Endophyte specific primers/probes could detect contamination of AR37 seeds with other endophytes down to a level of 3-25%. We demonstrated that it is possible to quantify different endophyte strains simultaneously using multiplex PCR. This method has potential applications in management of endophytes in pastures and in fundamental research into this important plant-microbe symbiosis." |
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| Language: | English |
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| References: | 42 |
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| Note: | Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Zhou, Y., R. E. Bradshaw, R. D. Johnson, D. E. Hume, W. R. Simpson, and J. Schmid. 2014. Detection and quantification of three distinct Neotyphodium lolii endophytes in Lolium perenne by real time PCR of secondary metabolite genes. Fungal Biology. 118(3):p. 316-324. |
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| DOI: 10.1016/j.funbio.2014.01.003 |
| Web URL(s):
http://www.sciencedirect.com/science/article/pii/S1878614614000105
Last checked: 03/25/2014
Access conditions: Item is within a limited-access website |
| 
MSU catalog number: b7174068 |
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