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| DOI: | 10.1016/j.cropro.2013.07.017 |
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| Web URL(s): | http://www.sciencedirect.com/science/article/pii/S0261219413001968
Last checked: 04/15/2014
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Koch, P. L.;
Stier, J. C.;
Senseman, S. A.;
Sobek, S.;
Kerns, J. P. |
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| Author Affiliation: | Koch: Department of Plant Pathology, University of Wisconsin-Madison; Stier: University of Tennessee-Knoxville and Department of Crop and Soil Science; Senseman: Department of Agriculture, Texas A&M; Sobek: Wisconsin Department of Agriculture, Trade, and Consumer Protection; Kerns: Department of Plant Pathology, North Carolina State University |
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| Title: | Modification of a commercially-available ELISA kit to determine chlorothalonil and iprodione concentration on golf course turfgrass |
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| Source: | Crop Protection. Vol. 54, December 2013, p. 35-42. |
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| Publishing Information: | Amsterdam, The Netherlands: Elsevier Science |
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| # of Pages: | 8 |
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| Keywords: | TIC Keywords: Case studies; Chlorothalonil; Enzyme-linked immunosorbent assay; Fungicide application; Fungicide efficacy; Gas chromatography; Iprodione
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| Trade Names: | SmartAssay ELISA kit |
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| Abstract/Contents: | "Repeated fungicide applications are often required for successful management of diseases on golf course turfgrass. Modification of existing commercially-available enzyme-linked immunosorbent assays (ELISA) for analyzing fungicide concentration on turfgrass would allow for more direct research of fungicide fate under varying environmental conditions. Our objective was to modify Horiba SmartAssay® ELISA kit procedures to increase their efficiency and practicality for analyzing iprodione and chlorothalonil from large numbers of turfgrass samples. Both fungicides were applied to creeping bentgrass (Agrostis stolonifera L.) turfgrass maintained under fairway conditions. The ELISA results were compared to fungicide concentrations obtained using gas chromatography/electron capture detection (GC/ECD). Iprodione concentrations from turfgrass 1 h following application using ELISA averaged 371.3 μg g-1 turfgrass, whereas GC/ECD averaged 151.2 μg g-1. Chlorothalonil concentrations from turfgrass 1 h following application using ELISA averaged 1883.7 μg g-1, compared to average concentrations of 553.1 μg g-1 using GC/ECD. Despite the higher fungicide concentrations observed using the ELISA method, the modified Horiba SmartAssay® kits yielded consistent results at a fraction of the cost, time, and skill set of using gas chromatographic methods. The modified ELISA protocol could be used to gain a further understanding of fungicide fate in turfgrass systems under varying environmental conditions, potentially improving the efficiency of future fungicide applications." |
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| Language: | English |
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| References: | 27 |
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| Note: | Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Koch, P. L., P. L. Koch, J. C. Stier, S. A. Senseman, S. Sobek, and J. P. Kerns. 2013. Modification of a commercially-available ELISA kit to determine chlorothalonil and iprodione concentration on golf course turfgrass. Crop Prot. 54:p. 35-42. |
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| DOI: 10.1016/j.cropro.2013.07.017 |
| Web URL(s):
http://www.sciencedirect.com/science/article/pii/S0261219413001968
Last checked: 04/15/2014
Access conditions: Item is within a limited-access website |
| 
MSU catalog number: b2337143 |
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