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Web URL(s): | http://www.nrcresearchpress.com/doi/pdf/10.1139/b92-057 Last checked: 09/29/2015 Requires: PDF Reader Access conditions: Item is within a limited-access website |
Publication Type:
| Refereed |
Author(s): | Koga, Hironori;
Tsukiboshi, Takao;
Uematsu, Tsutomu |
Author Affiliation: | Department of Environment, National Grassland Research Institute, Japan |
Title: | Application of an osmium-maceration technique to observe plant--microbe interfaces of Italian ryegrass and crown rust fungi by scanning electron microscopy |
Source: | Canadian Journal of Botany. Vol. 70, No. 2, February 1992, p. 438-442. |
Publishing Information: | Vancouver, British Columbia: The National Research Council of Canada. |
# of Pages: | 5 |
Related Web URL: | http://www.nrcresearchpress.com/doi/abs/10.1139/b92-057 Last checked: 09/29/2015 Notes: English abstract only |
Keywords: | TIC Keywords: Lolium multiflorum; Puccinia coronata var. coronata; Scanning electron microscopy
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Abstract/Contents: | "The osmium-maceration technique (osmium-DMSO-osmium method) was used to observe intracellular structures of Italian ryegrass leaves infected with crown rust fungus (Puccinia coronata Corda var. coronata) by scanning electron microscopy. Prolonged maceration with dilute osmium removed the cytoplasmic matrix, and intracellular structures were structures of host and parasite cells were disclosed in a bold relief. The three-dimensional configuration of nuclei, mitochondria, the endoplasmic reticulum, and other structures of host and parasite cells were visible." |
Language: | English |
References: | 8 |
Note: | Abstract also appears in French Pictures, b/w |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Koga, H., T. Tsukiboshi, and T. Uematsu. 1992. Application of an osmium-maceration technique to observe plant--microbe interfaces of Italian ryegrass and crown rust fungi by scanning electron microscopy. Can. J. Bot. 70(2):p. 438-442. |
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| Web URL(s): http://www.nrcresearchpress.com/doi/pdf/10.1139/b92-057 Last checked: 09/29/2015 Requires: PDF Reader Access conditions: Item is within a limited-access website |
| MSU catalog number: QK 1 .C3 |
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