Full TGIF Record # 23991
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Web URL(s):http://www.nrcresearchpress.com/doi/pdf/10.1139/b92-057
    Last checked: 09/29/2015
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    Access conditions: Item is within a limited-access website
Publication Type:
i
Refereed
Author(s):Koga, Hironori; Tsukiboshi, Takao; Uematsu, Tsutomu
Author Affiliation:Department of Environment, National Grassland Research Institute, Japan
Title:Application of an osmium-maceration technique to observe plant--microbe interfaces of Italian ryegrass and crown rust fungi by scanning electron microscopy
Source:Canadian Journal of Botany. Vol. 70, No. 2, February 1992, p. 438-442.
Publishing Information:Vancouver, British Columbia: The National Research Council of Canada.
# of Pages:5
Related Web URL:http://www.nrcresearchpress.com/doi/abs/10.1139/b92-057
    Last checked: 09/29/2015
    Notes: English abstract only
Keywords:TIC Keywords: Lolium multiflorum; Puccinia coronata var. coronata; Scanning electron microscopy
Abstract/Contents:"The osmium-maceration technique (osmium-DMSO-osmium method) was used to observe intracellular structures of Italian ryegrass leaves infected with crown rust fungus (Puccinia coronata Corda var. coronata) by scanning electron microscopy. Prolonged maceration with dilute osmium removed the cytoplasmic matrix, and intracellular structures were structures of host and parasite cells were disclosed in a bold relief. The three-dimensional configuration of nuclei, mitochondria, the endoplasmic reticulum, and other structures of host and parasite cells were visible."
Language:English
References:8
Note:Abstract also appears in French
Pictures, b/w
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Koga, H., T. Tsukiboshi, and T. Uematsu. 1992. Application of an osmium-maceration technique to observe plant--microbe interfaces of Italian ryegrass and crown rust fungi by scanning electron microscopy. Can. J. Bot. 70(2):p. 438-442.
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Web URL(s):
http://www.nrcresearchpress.com/doi/pdf/10.1139/b92-057
    Last checked: 09/29/2015
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
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