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Web URL(s): | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC546028/?page=2 Last checked: 5/7/2014 Notes: Item is within a single large file http://www.ncbi.nlm.nih.gov/pmc/articles/PMC546028/pdf/plntphys00218-0013.pdf#page=2 Last checked: 5/7/2014 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Refereed |
Author(s): | Chen, T. M.;
Brown, R. H.;
Black, C. C. Jr. |
Author Affiliation: | Department of Agronomy and Department of Biochemistry, University of Georgia, Athens, Georgia |
Title: | Biochemical activity of chloroplasts isolated from species with high photosynthetic capacity |
Section: | Chloroplast structure Other records with the "Chloroplast structure" Section
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Source: | Plant Physiology. Vol. 44, No. Supplement, 1969, p. 11. |
Publishing Information: | Rockville, MD: American Society of Plant Physiologists |
# of Pages: | 1 |
Keywords: | TIC Keywords: Chloroplasts; Cynodon dactylon; Photophosphorylation; Photosynthesis; Physiological processes; Temperature response
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Abstract/Contents: | "Bermudagrass (Cynodon dactylon, L.) has been reported to have an optimum temperature for growth near 35°C, a high rate of CO2 fixation in intense light (50-70 mg CO2 fixed dm-2 hr-1) and a low CO2 compensation concentration (> 5 ppm). Bermudagrass chloroplasts active in photophosphorylation and electron transport have been successfully isolated in 0.02 M tris-HCl and 0.35 M NaCl at pH 8.0. The optimum levels of reagents in photophosphorylation in umoles per ml reaction mixture are as follows: tricine pH 8.0 50, MgCl2 2, ADP 0.25, Pi 2, phenazine methosulfate 0.005 to 0.01, and ferricyanide 0.05 to 0.1. The electron transport reactions, e.g., NADP+, ferricyanide, cytochrome c and FMN reduction appear to be similar to spinach and are coupled with a similar stoichiometry to photophosphorylation. An apparent Km value was obtained for ADP of 2.5 Ξ 10-5 MI and for Pi of 5 Ξ 10-5 MAI during photophosphorylation. The molar ratio of cyclic/noncyclic photophosphorylation with bermudagrass was higher than spinach and pokeweed chloroplasts. The high cyclic photophosphorylation and the high affinity for cofactors of photophosphorylation indicate that these chloroplasts could supply the ATP required for CO2 fixation in bermudagrass leaves." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Chen, T. M., R. H. Brown, and C. C. Jr. Black. 1969. Biochemical activity of chloroplasts isolated from species with high photosynthetic capacity. Plant Physiol. 44(Supplement):p. 11. |
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| Web URL(s): http://www.ncbi.nlm.nih.gov/pmc/articles/PMC546028/?page=2 Last checked: 5/7/2014 Notes: Item is within a single large file http://www.ncbi.nlm.nih.gov/pmc/articles/PMC546028/pdf/plntphys00218-0013.pdf#page=2 Last checked: 5/7/2014 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: b2215281a |
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