Full TGIF Record # 241403
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DOI:10.1094/PHYTO-97-6-0717
Web URL(s):http://apsjournals.apsnet.org/doi/pdfplus/10.1094/PHYTO-97-6-0717
    Last checked: 05/13/2014
    Requires: PDF Reader
Publication Type:
i
Refereed
Author(s):Barnes, C. W.; Szabo, L. J.
Author Affiliation:United States Department of Agriculture-Agricultural Research Service, Cereal Disease Laboratory, University of Minnesota, Department of Plant Pathology, St. Paul
Title:Detection and identification of four common rust pathogens of cereals and grasses using real-time polymerase chain reaction
Source:Phytopathology. Vol. 97, No. 6, June 2007, p. 717-727.
Publishing Information:Lancaster, Pennsylvania: The Society Intelligencer Printing Company for The American Phytopathological Society
# of Pages:11
Related Web URL:http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-97-6-0717
    Last checked: 05/13/2014
    Notes: Abstract only
Keywords:TIC Keywords: Disease evaluation; Disease identification; Disease surveys; Pastures; Polymerase chain reaction; Puccinia; Puccinia graminis subsp. graminis; Puccinia recondita; Puccinia striiformis; Rusts
Abstract/Contents:"Puccinia spp. are widespread pathogens of cereals and grasses that annually cause significant yield losses worldwide, especially in barley, oat, and wheat. Urediniospore morphology and early symptom development have limited usefulness for distinguishing Puccinia spp. Therefore, we developed real-time polymerase chain reaction assays for rapid detection of the four rust pathogen species, Puccinia graminis (Pers.:Pers.), P. striiformis (Westend.), P. triticina (Eriks.), and P. recondita (Roberge ex Desmaz.). Duplex assays were constructed for the nuclear rDNA gene, using the variable internal transcribed spacer 1 (ITS1) region to distinguish between species, and the conserved 28S region as an internal control. Species-specific ITS1 primer/probe sets were highly specific and could detect <1 pg of DNA. The species-specific primer/probe sets showed positive results over a linear range of DNA five orders of magnitude or greater. Specificity of the assays was tested using multiple collections representing a range of races and formae speciales within a species. Additionally, assay specificity was evaluated by testing a range of other grass rust pathogens, as well as other fungi. The 28S primer/probe combination was successful in detecting all Puccinia spp. tested within the duplex assays, validating the integrity of each assay. Finally, the assays were used to identify unknown rust fungi infecting pasture grasses."
Language:English
References:40
Note:Pictures, b/w
Tables
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Barnes, C. W., and L. J. Szabo. 2007. Detection and identification of four common rust pathogens of cereals and grasses using real-time polymerase chain reaction. Phytopathology. 97(6):p. 717-727.
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DOI: 10.1094/PHYTO-97-6-0717
Web URL(s):
http://apsjournals.apsnet.org/doi/pdfplus/10.1094/PHYTO-97-6-0717
    Last checked: 05/13/2014
    Requires: PDF Reader
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