Full TGIF Record # 242006
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DOI:10.1071/AP08077
Web URL(s):https://link.springer.com/content/pdf/10.1071/AP08077.pdf
    Last checked: 10/05/2017
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Publication Type:
i
Refereed
Author(s):Dracatos, P. M.; Dobrowolski, M. P.; Lamb, J.; Olle, R. S.; Gendall, A. R.; Cogan, N. O. I.; Smith, K. F.; Forster, J. W.
Author Affiliation:Dracatos, Dobrowolski, Lamb, Olle, Cogen, Smith, and Forster: Molecular Plant Breeding Cooperative Research Centre; Dracatos, Cogan, and Forster: Department of Primary Industries, Biosciences Research Division, Vicotiran AgriBiosciences Centre, La Trobe University Research and Development Park; Dracatos and Gendall: Department of Botany, Faculty of Science, Technology and Engineering, La Trobe University, Bundoora, Vic.; Dobrowolski, Lamb, Olle, and Smith: Department of Primary Industries, Biosciences Research Division, Hamilton Centre, Hamilton, Vic., Australia
Title:Development of genetically homogenised populations of the crown rust pathogen (Puccinia coronata f. sp. lolii) for disease trait dissection in perennial ryegrass (Lolium perenne L.)
Source:Australasian Plant Pathology. Vol. 38, No. 1, January 2009, p. 55-62.
# of Pages:8
Publishing Information:Clayton, Vic. : Australian Plant Pathology Society
Related Web URL:https://link.springer.com/article/10.1071/AP08077
    Last checked: 10/05/2017
    Notes: Abstract only
Keywords:TIC Keywords: Genetic analysis; Inoculum; Lolium perenne; Puccinia coronata var. coronata; Simple sequence repeats
Abstract/Contents:"Genetic analysis of host resistance to the crown rust pathogen of ryegrasses has traditionally involved the use of composite urediniospore collections, which may contain multiple genotypes and virulence races. Consequently, discrimination between major gene responses to alternative races and quantitative resistance has proved challenging for disease trait-dissection studies. A method of propagation on detached leaves has been used to derive genetically homogenised crown rust pathogen lines from bulked spore collections obtained from five distinct Australian locations. Spores from individual pustules were repeatedly reinoculated onto fresh leaves. Amplicon complexity was assessed at the conclusion of each cycle using crown rust pathogen expressed sequence tag (EST)-derived simple sequence repeat (SSR) primer pairs. The mean number of amplification products per isolate declined over successive rounds of reinfection. The number of rounds of sequential infection required for decay to stable levels was dependent on the diversity present within the bulked spore collection. Large-scale propagation of the putative homogenised populations developed in the present study may provide inoculum for infection studies to allow quantitative trait loci mapping of specific gene-for-gene responses. The methodology has significant applications for the pyramiding of resistance genes specific to diverse crown rust pathogen genotypes during cultivar development and for pathotyping of existing elite germplasm. This approach is also applicable to other Puccinia species."
Language:English
References:27
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Dracatos, P. M., M. P. Dobrowolski, J. Lamb, R. S. Olle, A. R. Gendall, N. O. I. Cogan, et al. 2009. Development of genetically homogenised populations of the crown rust pathogen (Puccinia coronata f. sp. lolii) for disease trait dissection in perennial ryegrass (Lolium perenne L.). Australas. Plant Pathol. 38(1):p. 55-62.
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DOI: 10.1071/AP08077
Web URL(s):
https://link.springer.com/content/pdf/10.1071/AP08077.pdf
    Last checked: 10/05/2017
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
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