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| DOI: | 10.2307/3760703 |
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| Web URL(s): | http://www.jstor.org/stable/view/3760703
Last checked: 06/12/2014
Access conditions: Item is within a limited-access website
http://www.jstor.org/stable/pdfplus/3760703.pdf
Last checked: 06/12/2014
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| Publication Type:
| Refereed |
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| Author(s): | Carbone, Ignazio;
Kohn, Linda M. |
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| Author Affiliation: | Department of Botany, University of Toronto, Erindale College, Mississauga, Ontario, Canada |
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| Title: | Ribosomal DNA sequence divergence within internal transcribed spacer 1 of the Sclerotiniaceae |
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| Section: | Molecular biology
Other records with the "Molecular biology" Section
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| Source: | Mycologia. Vol. 85, No. 3, May/June 1993, p. 415-427. |
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| Publishing Information: | Lancaster, Pennsylvania: New Era Print Co. for the New York Botanical Garden |
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| # of Pages: | 13 |
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| Related Web URL: | http://www.jstor.org/stable/info/3760703
Last checked: 06/12/2014
Notes: Abstract only |
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| Keywords: | TIC Keywords: Ascomycota; Genetic analysis; Genetic characterization; Genetic markers; Pathogenic fungi; Phylogenetic analysis; Sclerotinia sclerotiorum; Sclerotiniaceae; Stromatinia cepivora; Taxonomic families; Taxonomy
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| Abstract/Contents: | "Based on morphological and immunological studies, we hypothesize that there are two lineages within the Sclerotiniaceae, a family of plant-infecting ascomycetes in the order Helotiales: 1) genera producing sclerotia, which are tuberlike, melanized masses of hyphae, and 2) genera producing substratal stromata, which are mats of compact hyphae that incorporate plant tissues. We sequenced the Internal Transcribed Spacer (ITS 1), defined by primers ITS 1 and 2, in 43 isolates: 29 sclerotial isolates (19 species in 9 genera), 11 substratal isolates (8 species in 4 genera), and 3 outgroup isolates in the Leotiaceae (3 species in 3 genera). Direct, double-stranded sequencing yielded ca 170 bases for sclerotial isolates and ca 200 bases for substratal and outgroup isolates. MACVECTOR and MULTALIN were used for global alignment, and multiple alignment with hierarchical clustering, respectively. The Internal Transcribed Spacer showed close similarity among most of the sclerotial taxa (76 to 100% similarity to Sclerotinia sclerotiorum). This supports our hypothesis that a sclerotial lineage exists and suggests that this lineage has evolved relatively recently. Isolates of the asexual (mitotic) species Sclerotium cepivorum showed 98% similarity to those of the genus Sclerotinia. Sequence divergence was greater (45 to 65% similarity to S. sclerotiorum) amongst the substratal taxa and our outgroups. Parsimony analysis produced one statistically strongly supported tree for a group of species in the genus Rutstroemia, including Sclerotinia homoeocarpa. Although such subclusters of species can be distinguished using parsimony analysis, we conclude that a substratal lineage cannot be discerned based on sequence data from the ITS. Among these more distantly related taxa, including some substratal ingroup taxa and the outgroup taxa, ITS 1 is saturated with changes and shows relatively equal dissimilarity. The variation observed in the ITS does not resolve among more distantly related taxa." |
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| Language: | English |
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| References: | 33 |
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| Note: | Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Carbone, I., and L. M. Kohn. 1993. Ribosomal DNA sequence divergence within internal transcribed spacer 1 of the Sclerotiniaceae. Mycologia. 85(3):p. 415-427. |
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| DOI: 10.2307/3760703 |
| Web URL(s):
http://www.jstor.org/stable/view/3760703
Last checked: 06/12/2014
Access conditions: Item is within a limited-access website
http://www.jstor.org/stable/pdfplus/3760703.pdf
Last checked: 06/12/2014
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: b2214983
MSU catalog number: b5343430 |
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