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| DOI: | 10.1080/07060661.2014.906505 |
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| Web URL(s): | http://www.tandfonline.com/doi/full/10.1080/07060661.2014.906505
Last checked: 07/14/2014
Access conditions: Item is within a limited-access website
http://www.tandfonline.com/doi/pdf/10.1080/07060661.2014.906505
Last checked: 07/14/2014
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Rioux, Renee A.;
Van Ryzin, Benjamin J.;
Kerns, James P. |
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| Author Affiliation: | Rioux: Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI; Van Ryzin and Kerns: Department of Plant Pathology, North Carolina State University, Raleigh, NC |
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| Title: | Development of a semi-selective medium for improved isolation of the turfgrass dollar spot pathogen Sclerotinia homoeocarpa from host tissues |
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| Section: | Soilborne pathogens
Other records with the "Soilborne pathogens" Section
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| Source: | Canadian Journal of Plant Pathology. Vol. 36, No. 2, 2014, p. 235-245. |
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| Publishing Information: | [Canada]: Canadian Phytopathological Society |
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| # of Pages: | 11 |
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| Related Web URL: | http://www.tandfonline.com/doi/abs/10.1080/07060661.2014.906505
Last checked: 07/14/2014
Notes: English and French abstracts only |
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| Keywords: | TIC Keywords: Disease identification; Dollar spot; Fungicide evaluation; Growing media; Sclerotinia homoeocarpa
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| Abstract/Contents: | "Dollar spot, caused by Sclerotinia homoeocarpa, is one of the most economically devastating diseases of amenity turfgrasses worldwide. The pathogen is readily isolated from active lesions, but detection from seed, dormant host tissue and other plant debris that may serve as a source of primary inoculum is difficult. A semi-selective medium was developed to enhance isolation of S. homoeocarpa. Various fungicides used on turfgrass, the pH indicator dye bromophenol blue, and two pH levels, were assessed for their effects on, and in the case of bromophenol blue, response to growth of S. homoeocarpa and contaminant fungi frequently isolated from field and seed samples. Amendment of the medium to pH 4 promoted growth of S. homoeocarpa in the absence of fungicides and enhanced pathogen growth relative to contaminant isolates on medium amended with 0.1 μg mL-1 triticonazole or 5 μg mL-1 azoxystrobin. The growth rate of S. homoeocarpa on these three media, as determined by in vitro radial growth assays, was consistent for many isolates representing the majority of S. homoeocarpa vegetative compatibility groups. Isolation efficiency on the pH-amended media was tested against antibiotic-amended potato dextrose agar from field samples and artificially inoculated turfgrass seed. In both cases, the medium amended to pH 4 and containing 5 μg mL-1 azoxystrobin significantly decreased contaminant growth with no adverse effects on recovery of S. homoeocarpa in comparison with antibiotic-amended PDA. The enhanced specificity of this medium will be a useful tool for selective detection of S. homoeocarpa and identification of sources of initial inoculum for dollar spot development." |
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| Language: | English |
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| References: | 44 |
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| Note: | Abstract also appears in French
Tables
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Van Ryzin, B. J., J. P. Kerns, and R. A. Rioux. 2014. Development of a semi-selective medium for improved isolation of the turfgrass dollar spot pathogen Sclerotinia homoeocarpa from host tissues. Can. J. Plant Pathol. 36(2):p. 235-245. |
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| DOI: 10.1080/07060661.2014.906505 |
| Web URL(s):
http://www.tandfonline.com/doi/full/10.1080/07060661.2014.906505
Last checked: 07/14/2014
Access conditions: Item is within a limited-access website
http://www.tandfonline.com/doi/pdf/10.1080/07060661.2014.906505
Last checked: 07/14/2014
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: b2189612 |
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