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| DOI: | 10.1007/s11032-014-0044-0 |
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| Web URL(s): | https://link.springer.com/article/10.1007/s11032-014-0044-0
Last checked: 10/06/2017
Access conditions: Item is within a limited-access website
https://link.springer.com/content/pdf/10.1007%2Fs11032-014-0044-0.pdf
Last checked: 10/06/2017
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Birrer, Martina;
Kölliker, Roland;
Manzanares, Chloe;
Asp, Torben;
Studer, Bruno |
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| Author Affiliation: | Birrer, Manzanares and Studer: Forage Crop Genetics, Institute of Agricultural Sciences, ETH Zurich; Kölliker; Institute for Sustainability Sciences, ISS, Agroscope, Zurich, Switzerland; Asp: Department of Molecular Biology and Genetics, Faculty of Science and Technology, Aarhus University, Slagelse, Denmark |
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| Title: | A DNA marker assay based on high-resolution melting curve analysis for distinguishing species of the Festuca-Lolium complex |
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| Source: | Molecular Breeding. Vol. 34, No. 2, August 2014, p. 421-429. |
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| Publishing Information: | The Netherlands: Kluwer Academic Publishers |
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| # of Pages: | 9 |
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| Keywords: | TIC Keywords: Cultivar identification; DNA; Genetic markers; Lolium multiflorum; Lolium perenne; Polymerase chain reaction; Seed contamination; Single nucleotide polymorphism; Variety trials
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| Abstract/Contents: | "The grass breeding industry is interested in a fast and cheap method of identifying contamination in seeds of Italian and perennial ryegrass (Lolium perenne L. and L. multiflorum Lam., respectively). This study shows that high-resolution melting curve analysis in combination with an unlabelled probe assay is an effective method of detecting single nucleotide polymorphisms (SNPs) in diverse Italian and perennial ryegrass backgrounds. This method proved efficient in differentiating ryegrass species and reducing the effect of additional DNA sequence polymorphisms close to the target SNP on the melting curve profiles. For the identification of contamination in Italian and perennial ryegrass seed production, high-resolution melting curve analysis shows great potential, as it is a single closed-tube PCR reaction with an easy workflow, providing results in <2 h after DNA extraction." |
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| Language: | English |
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| References: | 19 |
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| Note: | Figures
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Birrer, M., R. Kölliker, C. Manzanares, T. Asp, and B. Studer. 2014. A DNA marker assay based on high-resolution melting curve analysis for distinguishing species of the Festuca-Lolium complex. Molecular Breeding. 34(2):p. 421-429. |
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| DOI: 10.1007/s11032-014-0044-0 |
| Web URL(s):
https://link.springer.com/article/10.1007/s11032-014-0044-0
Last checked: 10/06/2017
Access conditions: Item is within a limited-access website
https://link.springer.com/content/pdf/10.1007%2Fs11032-014-0044-0.pdf
Last checked: 10/06/2017
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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