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| DOI: | 10.21273/JASHS.139.5.547 |
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| Web URL(s): | https://journals.ashs.org/jashs/view/journals/jashs/139/5/article-p547.xml
Last checked: 04/30/2020
https://journals.ashs.org/jashs/downloadpdf/journals/jashs/139/5/article-p547.xml
Last checked: 04/30/2020
Requires: PDF Reader |
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| Publication Type:
| Refereed |
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| Author(s): | Harris-Shultz, Karen R.;
Milla-Lewis, Susana;
Patton, Aaron J.;
Kenworthy, Kevin;
Chandra, Ambika;
Waltz, F. Clint;
Hodnett, George L.;
Stelly, David M. |
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| Author Affiliation: | Harris-Shultz: USDA-ARS, Crop Genetics and Breeding Unit, Tifton; Waltz: Crop and Soil Sciences, Georgia Experiment Station, The University of Georgia, Griffin, GA; Milla-Lewis: Department of Crop Science, North Carolina State University, Raleigh, NC; Patton: Department of Agronomy, Purdue University, West Lafayette, IN; Kenworthy: Agronomy Department, University of Florida, Gainesville, FL; Chandra: Texas AgriLife Research and Extension Center, Texas A&M University System, Dallas; Hodnett and Stelly: Department of Soil and Crop Sciences, Texas A&M University, College Station, TX |
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| Title: | Detection of DNA and ploidy variation within vegetatively propagated zoysiagrass cultivars |
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| Source: | Journal of The American Society for Horticultural Science. Vol. 139, No. 5, September 2014, p. 547-552. |
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| Publishing Information: | Alexandria, Virginia: American Society for Horticultural Science |
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| # of Pages: | 12 |
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| Keywords: | TIC Keywords: Contamination; Cross pollination; Cultivar evaluation; Genetic variability; Hybridization; Self-pollination; Warm season turfgrasses; Zoysia
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| Cultivar Names: | Emerald; Diamond; Empire; JaMur; Meyer; Zeon; Geo; Atlantic |
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| Abstract/Contents: | "Zoysiagrass (Zoysia sp.) is used as a warm-season turfgrass for lawns, parks, and golf courses in the warm, humid and transitional climatic regions of the United States. Zoysiagrass is an allotetraploid species (2n = 4x = 40) and some cultivars are known to easily self- and cross-pollinate. Previous studies showed that genetic variability in the clonal cultivars Emerald and Diamond was likely the result of contamination (seed production or mechanical transfer) or mislabeling. To determine the extent of genetic variability of vegetatively propagated zoysiagrass cultivars, samples were collected from six commercially available zoysiagrass cultivars (Diamond, Emerald, Empire, JaMur, Meyer, Zeon) from five states (Arkansas, Florida, Georgia, North Carolina, Texas). Two of the newest cultivar releases (Geo and Atlantic) were to serve as outgroups. Where available, one sample from university research plots and two samples from sod farms were collected for each cultivar per state. Forty zoysiagrass simple sequence repeat (SSR) markers and flow cytometry were used to compare genetic and ploidy variation of each collected sample to a reference sample. Seventy-five samples were genotyped and an unweighted pair group method with arithmetic mean clustering revealed four groups. Group I (Z. Japonica) included samples of 'Meyer' and Empire11 ('Empire' sample at location #11), Group II (Z. japonica ^DD#X Z. pacifica) included samples of 'Emerald' and 'Geo', Group III (Z. matrella) included samples of 'Diamond' and 'Zeon', and Group IV (Z. japonica) consisted of samples from 'Empire', 'JaMur', 'Atlantic', and Meyer3 ('Meyer' at sample location #3). Samples of 'Empire', 'Atlantic', and 'JaMur' were indistinguishable with the markers used. Four samples were found to have alleles different from the respective reference cultivar, including two samples of 'Meyer', one sample of 'Empire', and one sample of 'Emerald'. Three of these samples were from Texas and one of these samples was from Florida. Three of the four samples that were different from the reference cultivar were university samples. In addition, one sample, Empire11, was found to be an octoploid (2n = 8x = 80). For those samples that had a fingerprint different from the reference cultivar, contamination, selfing, and/or hybridization with other zoysiagrasses may have occurred." |
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| Language: | English |
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| References: | 41 |
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| Note: | Supplemental tables appear on separate unnumbered pages
Pictures, b/w
Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Harris-Shultz, K. R., S. Milla-Lewis, A. J. Patton, K. Kenworthy, A. Chandra, F. C. Waltz, et al. 2014. Detection of DNA and ploidy variation within vegetatively propagated zoysiagrass cultivars. J. Am. Soc. Hortic. Sci. 139(5):p. 547-552. |
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| DOI: 10.21273/JASHS.139.5.547 |
| Web URL(s):
https://journals.ashs.org/jashs/view/journals/jashs/139/5/article-p547.xml
Last checked: 04/30/2020
https://journals.ashs.org/jashs/downloadpdf/journals/jashs/139/5/article-p547.xml
Last checked: 04/30/2020
Requires: PDF Reader |
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MSU catalog number: b2221587a |
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