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DOI: | 10.3732/ajb.1000254 |
Web URL(s): | http://www.amjbot.org/content/97/10/e99.full Last checked: 11/12/2014 Access conditions: Item is within a limited access website http://www.amjbot.org/content/97/10/e99.full.pdf Last checked: 11/12/2014 Requires: PDF Reader Access conditions: Item is within a limited-access website |
Publication Type:
| Refereed |
Author(s): | Jewell, Margaret C.;
Frere, Celine H.;
Prentis, Peter J.;
Lambrides, Christopher J.;
Godwin, Ian D. |
Author Affiliation: | School of Land, Crop and Food Sciences, The University of Queensland, Brisbane, QLD, Australia |
Title: | Characterization and multiplexing of EST-SSR primers in Cynodon (Poaceae) species |
Section: | AJB primer notes & protocols in the plant sciences Other records with the "AJB primer notes & protocols in the plant sciences" Section
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Source: | American Journal of Botany. Vol. 97, No. 10, October 2010, p. e99-e101. |
Publishing Information: | Columbus, Ohio: Botanical Society of America |
# of Pages: | 3 |
Related Web URL: | http://www.amjbot.org/content/97/10/e99.abstract Last checked: 11/12/2014 Notes: Abstract only |
Keywords: | TIC Keywords: Cynodon; Genetic characterization; Genetic diversity; Polyploidy; Simple sequence repeats
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Geographic Terms: | Australia |
Abstract/Contents: | "Premise of the study: Cynodon species are multiple-use grasses that display varying levels of adaptation to biotic and abiotic stress. Previously identified EST-SSR primers were characterized and multiplexed to assess the level of genetic diversity present within a collection of almost 1200 Cynodon accessions from across Australia. Methods and Results: Two multiplex reactions were developed comprising a total of 16 EST-SSR markers. All SSR markers amplified across different Cynodon species and different levels of ploidy. The number of alleles ranged from one to eight per locus and the total number of alleles for the germplasm collection was 79. Conclusions: The 16 markers show sufficient variation for the characterization of Cynodon core collections and analysis of population genetic diversity in Cynodon grasses." |
Language: | English |
References: | 15 |
Note: | Tables |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Jewell, M., C. H. Frere, P. J. Prentis, C. J. Lambrides, and I. D. Godwin. 2010. Characterization and multiplexing of EST-SSR primers in Cynodon (Poaceae) species. Amer. J. Bot. 97(10):p. e99-e101. |
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| DOI: 10.3732/ajb.1000254 |
| Web URL(s): http://www.amjbot.org/content/97/10/e99.full Last checked: 11/12/2014 Access conditions: Item is within a limited access website http://www.amjbot.org/content/97/10/e99.full.pdf Last checked: 11/12/2014 Requires: PDF Reader Access conditions: Item is within a limited-access website |
| MSU catalog number: QK 1 .B345 |
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