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| DOI: | 10.1094/PHYTO-104-11-S3.1 |
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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-104-11-S3.1#page=75
Last checked: 11/25/2014
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Martinez-Espinoza, A. D.;
Alexander, R.;
Dutta, B. |
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| Author Affiliation: | Martinez-Espinoza and Alexander: University of Georgia, Griffin; Dutta: University of Georgia, Tifton, GA |
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| Title: | Incidence of bacterial decline of bentgrass in Georgia |
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| Section: | 2014 APS-CPS Joint Meeting abstracts of presentations
Other records with the "2014 APS-CPS Joint Meeting abstracts of presentations" Section
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| Meeting Info.: | Minneapolis, Minnesota: August 9-13, 2014 |
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| Source: | Phytopathology. Vol. 104, No. 11S, November 2014, p. S3.75. |
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| Publishing Information: | Lancaster, Pennsylvania: The Society Intelligencer Printing Company for The American Phytopathological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Acidovorax avenae; Agrostis stolonifera; Bacterial diseases; Disease resistance; Disease susceptibility; Enzyme-linked immunosorbent assay
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| Abstract/Contents: | "Diseased bentgrass (Agrostis palustris) plants were submitted to the UGA Plant Disease Clinic from a golf course in northeast GA. Symptoms were unique and included an abnormal plant elongation, general yellowing, wilt, and necrosis. Bacteria were found associated with the symptomatic tissue. The causal agent was determined to be Acidovorax avenea subsp avenea (Aaa). Here, we report a spatial and temporal incidence of this bacterial disease. Suspected bentgrass diseased samples collected in northeast Georgia, as well as samples from two additional sites located in north and northwest parts of the state, tested positive for Aaa using specific antibodies in ELISA. All of the selected colonies, which tested positive using ELISA, had 96-99% similarity to Aaa using BiologTM. Universal primers were used to amplify the 16S rRNA gene from four isolates and sequencing of amplified product showed 97 to 99% nucleotide similarity to Aaa. Further confirmation was achieved by amplifying bentgrass isolates with Aaa-specific primers in a nested PCR assay. The bacterial isolates were inoculated into healthy bentgrass cv Penncross plants. Healthy plants were successfully infected with Aaa when inoculated plants were incubated at high humidity (RH 90-100%) and high temperatures (30-35°C). Re-isolated bacterial colonies were characterized as Aaa using morphological features, ELISA specific antibodies and species-specific DNA primers." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Martinez-Espinoza, A. D., R. Alexander, and B. Dutta. 2014. Incidence of bacterial decline of bentgrass in Georgia. Phytopathology. 104(11S):p. S3.75. |
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| DOI: 10.1094/PHYTO-104-11-S3.1 |
| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-104-11-S3.1#page=75
Last checked: 11/25/2014
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: b2219736a |
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Digitally in TIC by file name: phytp2014novpres |
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