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| DOI: | 10.1094/PHYTO-104-11-S3.160 |
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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-104-11-S3.160#page=1
Last checked: 12/01/2014
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Beirn, L. A.;
Schmid, C. J.;
Hempfling, J. W.;
Murphy, J. A.;
Clarke, B. B.;
Crouch, J. A. |
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| Author Affiliation: | Beirn, Schmid, Hempfling, Murphy, and Clarke: Rutgers University, New Brunswick, NJ; Crouch: USDA-ARS, Beltsville, MD |
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| Title: | Metagenomic insights into nitrogen and potassium impacts on microbial community structure in Poa annua putting green turfgrass |
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| Section: | 2014 Potomac Division meeting abstracts
Other records with the "2014 Potomac Division meeting abstracts" Section
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| Meeting Info.: | Annapolis, Maryland: March 12-14, 2014 |
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| Source: | Phytopathology. Vol. 104, No. 11S, November 2014, p. S3.160. |
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| Publishing Information: | Lancaster, Pennsylvania: The Society Intelligencer Printing Company for The American Phytopathological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Microorganisms; Nitrogen; Poa annua; Polymerase chain reaction; Potassium; Rhizosphere
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| Abstract/Contents: | "Nitrogen (N) and potassium (K) fertility can have a significant impact on turfgrass diseases. Questions about whether these nutrients affect microbial community structure in the turfgrass rhizosphere remain unanswered. The objectives of this study were to evaluate the composition, diversity, and distribution of rhizosphere microorganisms in Poa annua (annual bluegrass) turf maintained as a putting green. Three 15.9 mm diameter × 50.8 mm long soil cores were sampled from four replicated plots receiving: (1) an intermediate N rate (132 kg N ha-1 yr-1); (2) K (200 kg K2O ha-1 yr-1); (3) N+K (1:1, N:K molar-adjusted ratio; 132 kg N ha-1 yr-1 and 200 kg K2O ha-1 yr-1); (4) a low N rate, (100 kg N ha-1 yr-1); or (5) a high N rate, (200 kg N ha-1 yr-1) (total samples = 60). N and K were applied as urea [CO(NH2)2] and potassium chloride (KCl), respectively. Organism-specific DNA regions were PCR amplified from fungi (ITS), bacteria and archaea (rDNA 16s). Multiplexed next-generation sequencing on an Illumina platform generated 2.3 × 107 reads (avg. 1.38 × 105 seq/per sample; avg. length 331 bp). After barcode trimming, demultiplexing, and chimera removal, 7.2 × 105 OTUs (97% identity) were identified using the open source QIIME pipeline in Python. Alpha diversity measures from rarified datasets showed that species diversity was highest in low and high N treatments, while diversity was lowest in plots receiving the intermediate rate of N. Detrended correspondence analysis performed using the Vegan module in R revealed significant differences in archaeal and bacterial communities from plots receiving intermediate N, K, or intermediate N+K treatments, compared to those receiving low or high rates of N. A similar finding was observed for the fungal community, indicating that differing management practices appear to be affecting the microbial community structure in this study." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Beirn, L. A., C. J. Schmid, J. W. Hempfling, J. A. Murphy, B. B. Clarke, and J. A. Crouch. 2014. Metagenomic insights into nitrogen and potassium impacts on microbial community structure in Poa annua putting green turfgrass. Phytopathology. 104(11S):p. S3.160. |
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| DOI: 10.1094/PHYTO-104-11-S3.160 |
| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-104-11-S3.160#page=1
Last checked: 12/01/2014
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: b2219736a |
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