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| DOI: | 10.1094/PHYTO-104-11-S3.165 |
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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-104-11-S3.165#page=10
Last checked: 12/01/2014
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Ostrander, J.;
Kennelly, M. |
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| Author Affiliation: | Kansas State University, Manhattan, KS |
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| Title: | Sensitivity of Kansas Sclerotinia homoeocarpa isolates to DMI fungicides |
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| Section: | 2014 North Central Division meeting abstracts
Other records with the "2014 North Central Division meeting abstracts" Section
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| Meeting Info.: | Madison, Wisconsin: June 11-13, 2014 |
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| Source: | Phytopathology. Vol. 104, No. 11S, November 2014, p. S3.174. |
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| Publishing Information: | Lancaster, Pennsylvania: The Society Intelligencer Printing Company for The American Phytopathological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Agrostis stolonifera; Application rates; DMI fungicides; Dollar spot; Fungicide evaluation; Growth analysis; Metconazole; Propiconazole; Sclerotinia homoeocarpa; Tebuconazole; Triticonazole
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| Abstract/Contents: | "Dollar spot, caused by Sclerotinia homoeocarpa, is a serious disease of intensively managed turfgrass such as creeping bentgrass (Agrostis stolonifera) in golf course fairways and putting greens, and fungicides are commonly used to maintain turfgrass at acceptable quality. S. homoeocarpa has shown resistance to sterol demethylation inhibitor (DMI) fungicides in many locations, but isolates in Kansas have not been tested. Our objectives were: (1) to determine the sensitivity of 74 Kansas S. homoeocarpa isolates to the DMI fungicides propiconazole, metconazole, tebuconazole, and triticonazole using in vitro mycelial growth assays; (2) to determine a single discriminatory concentration for each fungicide to be used in future testing; and (3) to examine the correlations among sensitivity to the different fungicides. Isolates were collected from 12 sites in Kansas, primarily golf course putting greens. In vitro growth assays were conducted at concentrations of 0, 0.001, 0.01, 0.05, 0.1 or 1.0 μg a.i. (active ingredient)/ml, and colony diameters were measured. For each fungicide concentration, the percent relative growth for each isolate was calculated by comparing the growth on amended media (GOA) compared to the growth on the nonamended control (GOC) by the equation (GOA/GOC) × 100. Using the log10 values of the fungicide concentration, regression was used to calculate the concentration to reduce growth by 50% (log10EC50). For all four fungicides, the log10EC50 values formed a unimodal curve but were not normally distributed. The mean EC50 values were 0.0163, 0.038, 0.0612, and 0.0994 μg a.i./ml for metconazole, propiconazole, tebuconazole, and triticonazole, respectively. Correlations were significant and positive for all pairwise comparisons of log10EC50 values. Regressions using discriminatory concentrations tested were significant. The most predictive concentrations were 0.01, 0.05, 0.05, and 0.10 μg a.i./ml for metconazole, propiconazole, tebuconazole, and triticonazole, respectively. This study provides a baseline for further monitoring and streamlining of resistance assays." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Ostrander, J., and M. Kennelly. 2014. Sensitivity of Kansas Sclerotinia homoeocarpa isolates to DMI fungicides. Phytopathology. 104(11S):p. S3.174. |
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| DOI: 10.1094/PHYTO-104-11-S3.165 |
| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-104-11-S3.165#page=10
Last checked: 12/01/2014
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: b2219736a |
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