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| DOI: | 10.1111/pbr.12207 |
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| Web URL(s): | http://onlinelibrary.wiley.com/doi/10.1111/pbr.12207/pdf
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http://onlinelibrary.wiley.com/doi/10.1111/pbr.12207/full
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Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Aguirre, Andrea Arias;
Studer, Bruno;
Do Canto, Javier;
Frei, Ursula;
Lübberstedt, Thomas |
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| Author Affiliation: | Aguirre, Do Canto, Frei, and Lübberstedt: Department of Agronomy, Iowa State University, Ames, IA; Studer: Forage Crop Genetics, Institute of Agricultural Sciences, ETH Zurich, Zurich, Switzerland; Do Canto: National Institute of Agricultural Research, INIA, Tacuarembo, Uruguay |
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| Title: | Validation of two models for self-incompatibility in autotetraploid perennial ryegrass using high resolution melting-based markers |
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| Source: | Plant Breeding. Vol. 133, No. 6, December 2014, p. 765-770. |
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| Publishing Information: | Berlin, Germany: P. Parey |
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| # of Pages: | 6 |
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| Related Web URL: | http://onlinelibrary.wiley.com/doi/10.1111/pbr.12207/abstract
Last checked: 12/04/2014
Notes: Abstract only |
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| Keywords: | TIC Keywords: Alleles; Cultivar evaluation; Genetic markers; Genotyping; Lolium perenne
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| Abstract/Contents: | "Perennial ryegrass (Lolium perenne L.) displays a two-locus gametophytic self-incompatibility (SI) system that remains intact at the tetraploid level. Two models are plausible for SI in autotetraploids. In Model I: both alleles at the S locus and both at the Z locus in diploid pollen matching the female genotype results in incompatibility. In Model II: only one allele at S and one at Z locus in diploid pollen matching the female results in incompatibility. The goals were to determine which of the models best explains SI in our autotetraploid ryegrass population and to evaluate the efficiency of high-resolution melting (HRM) genotyping for discriminating different iso-allelic genotypes. The progeny of a cross between two autotetraploids was characterized with three HRM-based markers co-segregating with Z. Segregation ratios were used to make inferences about the mode of action of the SI system. The observed segregation differed significantly (P < 0.001) from the expected under Model I, but not from the expected under Model II (P = 0.463). Thus, Model II explains SI in this population, and HRM is an efficient tool to distinguish different iso-allelic genotypic classes." |
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| Language: | English |
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| References: | 25 |
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| Note: | Figures
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Aguirre, A. A., B. Studer, J. Do Canto, U. Frei, and T. Lübberstedt. 2014. Validation of two models for self-incompatibility in autotetraploid perennial ryegrass using high resolution melting-based markers. Plant Breed. 133(6):p. 765-770. |
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| DOI: 10.1111/pbr.12207 |
| Web URL(s):
http://onlinelibrary.wiley.com/doi/10.1111/pbr.12207/pdf
Last checked: 12/04/2014
Requires: PDF Reader
Access conditions: Item is within a limited-access website
http://onlinelibrary.wiley.com/doi/10.1111/pbr.12207/full
Last checked: 12/04/2014
Access conditions: Item is within a limited-access website |
| 
MSU catalog number: b2174680 |
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