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| Publication Type:
| Report |
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| Author(s): | Lord, Fritz;
Konrad, Egli;
Sütterlin, Klaus;
Schmid, Tobias;
Fässler, Pius;
Glaubitz, Joachim |
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| Author Affiliation: | Lord: R&D Turf, COMPO EXPERT GmbH, Gildenstraße, Münster; Egli, Sütterlin, and Glaubitz: R&D Microbiology, Omya International AG, Oftringen; Schmid and Fässler; Otto Hauenstein Samen AG (OHS), Bahnhofstrasse, Rafz |
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| Title: | Erregern von Rasenkrankheiten mit Mikro Array Genchip Diagnose-System auf der Spur |
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| Source: | European Journal of Turfgrass Science. Vol. 45, No. 1, März 2014, p. 7-10. |
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| Publishing Information: | Bonn, Germany: Hortus Verlag |
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| # of Pages: | 4 |
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| Abstract/Contents: | "Conventional and optical analytical methods are generally used for the diagnostic of turf deseases. The identification of the pathogenic germs occurs after taking into consideration the morphological differentiators such as, for example, the form and the septation of the conidia. This method is time-consuming, labour-intensive, error-prone, and is very difficult to interpret, particularly for hybrid deseases. An exact identification of the pathogenic agent is one of the utmost importance when deciding the choice and the planning of the appropriate inspection and control measures. The PCR-method (Polymerase-Chain-Reaction) is a rather efficient and reliable diagnostic method on a molecular biological basis. The genetic material (DNA) is then duplicated in three times. Until now the PCR analysis has only allowed the testing of one or a few pathogenic germs which were suspected. By the way they were already anticipated. This analysis served merely to match the positive confirmation. Thanks these new tools out of the molecular biotechnology it is now possible to identify very quickly and reliably the pathogenic germs at the origin of most turf deseases. At first a very tiny quantity of grass near the root is taken as representative sample from which the DNA of the microorganisms contained therein is isolated. With the help of a particular micro-array-chip it is now possible to test a great number of pathogenic plant germs during one single scanning only. These results give important information on the phytosanitary status of the turf affected and can be used to plan an adequate treatment of these surfaces either directly of prophylactically. The DNA of the microorganisms obtained that way can be then copied when using special primers. With the ITS primer specially conceived for fungi it is possible to copy the DNA of every fungi contained in the sample. A new micro-array-chip was developed in close cooperation by Omya, COMPO Expert and OHS. This chip has a membrane with 100 microscopically small points. They bear the intrinsic DNA of 90 pathogenic germs, which are identified on the chip as noxious for turfs. Thanks this new method it is now possible to make the diagnosis of 90 pathogenic germs in one pass. Upon entry of the sample only one day work is necessary for the whole analysis. This innovative method of analysis offers greenkeepers new perspectives for a rash diagnosis of turf deseases and, at the same time, is an important tool for integrative plant protection. The first results obtained by this method appear on picture 7, table 1." |
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| Language: | German |
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| References: | 4 |
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| Note: | Summary appears as abstract
Summary also appears in English and French
Pictures, color
Figures
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Lord, F., E. Konrad, K. Sütterlin, T. Schmid, P. Fässler, and J. Glaubitz. 2014. Erregern von Rasenkrankheiten mit Mikro Array Genchip Diagnose-System auf der Spur. (In German) Rasen Turf Gazon. 45(1):p. 7-10. |
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