| |
| Web URL(s): | http://turf.rutgers.edu/research/abstracts/symposium2015.pdf#page=43
Last checked: 08/04/2015
Requires: PDF Reader
Notes: Item is within a single large file |
|---|
| Publication Type:
| Report |
|---|
| Author(s): | Zhao, Shuang;
Zhang, Lisa;
Clarke, Bruce B.;
Zhang, Ning |
|---|
| Author Affiliation: | Zhao, Clark and Zhang, N.: Deaprtment of Plant Biology and Pathology, Rutgers University; Zhang, L.: Yale University, New Haven, CT |
|---|
| Title: | Development and application of a TaqMan real-time PCR assay for rapid detection of the summer patch pathogen of turfgrass |
|---|
| Section: | Poster presentations
Other records with the "Poster presentations" Section
|
|---|
| Meeting Info.: | New Brunswick, New Jersey: January 16, 2015 |
|---|
| Source: | Proceedings of the Twenty-Fourth Annual Rutgers Turfgrass Symposium. 2015, p. 42. |
|---|
| Publishing Information: | New Brunswick, New Jersey: The Center for Turfgrass Science, Rutgers, The State University of New Jersey |
|---|
| # of Pages: | 1 |
|---|
| Keywords: | TIC Keywords: Application methods; Disease control; Disease identification; Magnaporthe poae; Pathogens; Polymerase chain reaction; Summer patch
|
|---|
| Abstract/Contents: | "In North America, one of the most important root diseases of Poa and Festuca turf is summer patch, caused by Magnaporthiopsis poae (=Magnaporthe poae). Detection and identification of M. poae in infected roots by conventional culture-based methods is difficult and time consuming. In turfgrass breeding and management evaluation studies, an important question to address is whether the better performance of some grasses is simply because of the absence of the pathogen in certain plots. In this study, a culture-independent TaqMan real-time PCR assay has been developed for M. poae that enables pathogen detection from the field samples within a few hours. The assay was validated with the target pathogen, closely related fungal species, and a number of other microorganisms that inhabit the same host and soil environment. This TaqMan real-time PCR assay is more sensitive (detecting as little as 3.88 pg genomic DNA of M. poae), rapid, and accurate compared to direct microscopic observation and isolation on a selective medium. The real-time PCR detection results correspond closely to visual assessments of disease severity in the field. Utilization of this assay in diagnostic laboratories will enable turfgrass managers to more quickly and effectively detect and potentially reduce fungicide usage through early and accurate identification of the pathogen." |
|---|
| Language: | English |
|---|
| References: | 0 |
|---|
| Note: | This item is an abstract only! |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Zhao, S., L. Zhang, B. B. Clarke, and N. Zhang. 2015. Development and application of a TaqMan real-time PCR assay for rapid detection of the summer patch pathogen of turfgrass. Proc. Rutgers Turfgrass Symp. p. 42. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=263842 |
| If there are problems with this record, send us feedback about record 263842. |
| Choices for finding the above item: |
|---|
| Web URL(s):
http://turf.rutgers.edu/research/abstracts/symposium2015.pdf#page=43
Last checked: 08/04/2015
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: b3696858 |
| Find from within TIC:
Digitally in TIC by file name: rutsy2015 |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
