Full TGIF Record # 264126
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Web URL(s):http://www.cropj.com/bustamante_9_7_2015_666_670.pdf
    Last checked: 08/11/2015
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Author(s):Bustamante, Fernanda de Oliveira; Rocha, Laiane Corsini; Santos, Nat√°lia de Souza; Silveira, Raphaela Aparecida Duarte; Nunes, Renata de Castro; Mittelmann, Andr√©a; Techio, V√Ęnia Helena
Author Affiliation:Bustamante, Rocha, Santos, Silveira, Nunes and Techio: Department of Biology, Federal University of Lavras, Academic Campus, Lavras, Minas Gerais State; Mittelmann: Researcher, Embrapa Dairy Cattle, Pelotas, RS, Brazil
Title:Analysis of nuclear DNA content and chromosome number for screening genotypes and crosses in annual ryegrass (Lolium multiflorum Lam.)
Source:Australian Journal of Crop Science. Vol. 9, No. 7, July 2015, p. 666-670.
# of Pages:5
Publishing Information:Australia: Southern Cross Publishing Group
Keywords:TIC Keywords: Breeding aims; Cultivar improvement; Cultivar profile; Cytogenetics; DNA; Genetic analysis; Genotypes; Laser flow cytometry; Lolium multiflorum; Polyploidy
Abstract/Contents:"Lolium multiflorum Lam. (annual ryegrass) is a forage [that] grows in temperate regions of the world. In South America, annual forms of L. multiflorum exist and have been used as forage for a long time in Argentina, Chile, Uruguay, and Brazil. This study determined the nuclear DNA content and chromosome number for screening genotypes and crosses in L. multiflorum from Brazil. Analyses to estimate nuclear DNA content by flow cytometry were performed in young leaves of four parental genotypes and eight offspring resulting from their crosses. To the chromosome counting, the slides were prepared by flame drying technique and Giemsa staining. The genotypes were classified in the following categories: diploid, triploid, and tetraploid with 14, 21 and 28 chromosomes, respectively. The average content of DNA in diploids ranged from 5.42pg to 5.85pg. A single triploid plant showed 8.30pg. In tetraploids, the average DNA content ranged from 10.21pg to 10.95pg. Thus, the combination of analyses by DNA quantification and chromosome counting prove to be efficient for screening and diagnosing ploidy level of parents and crosses in Lolium. This information may be used reliably for prior selection of genotypes for the breeding program."
Note:Pictures, b/w
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Bustamante, F. O., L. C. Rocha, N. S. Santos, R. A. D. Silveira, R. C. Nunes, A. Mittelmann, et al. 2015. Analysis of nuclear DNA content and chromosome number for screening genotypes and crosses in annual ryegrass (Lolium multiflorum Lam.). Australian Journal of Crop Science. 9(7):p. 666-670.
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