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| DOI: | 10.5660/WTS.2015.4.1.18 |
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| Web URL(s): | http://ocean.kisti.re.kr/downfile/volume/ksws/JDHHCQ/2015/v4n1/JDHHCQ_2015_v4n1_18.pdf
Last checked: 09/24/2015
Requires: PDF Reader |
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| Publication Type:
| Refereed |
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| Author(s): | Lee, Jeongran;
Kim, Chang-Seok;
Lee, In-Yong |
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| Author Affiliation: | National Academy of Agricultural Science, Iseo, Korea |
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| Title: | Molecular identification of Pooideae, Poaceae in Korea |
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| Source: | Weed and Turfgrass Science. Vol. 4, No. 1, March 2015, p. 18-25. |
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| Publishing Information: | Korea: The Korean Society of Weed Science and Turfgrass Society of Korea |
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| # of Pages: | 8 |
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| Related Web URL: | http://koreascience.or.kr/article/ArticleFullRecord.jsp?cn=JDHHCQ_2015_v4n1_18
Last checked: 09/24/2015
Notes: English abstract only |
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| Keywords: | TIC Keywords: DNA amplification; Genetic code; Genetic markers; Poaceae; Pooideae; Species identification
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| Geographic Terms: | Korea |
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| Abstract/Contents: | "A universal DNA barcoding for agricultural noxious weeds is a powerful technique for species identification without morphological knowledge, by using short sections of DNA from a specific region of the genome. Two standard barcode markers, chloroplast rbcL and matK, and a supplementary nuclear ribosomal Internal Transcribed Spacer (ITS) region were used to examine the effectiveness of the markers for Pooideae barcoding using 163 individuals of 29 taxa across 16 genera of Korean Pooideae. The rbcL and ITS revealed a good level of amplification and sequencing success while matK did not. Barcode gaps were 78.6% for rbcL, 96.2% for matK, and 91.7% for ITS, respectively. Resolving powers were 89.3% for rbcL, 92.3% for matK, and 79.1% for ITS. The matK obtained the best both barcode gap and resolving power. However, it should be considered not to employ matK for Pooideae barcode because of low rate of PCR amplification and sequencing success. As a single DNA marker, rbcL and ITS were reasonable for Pooideae barcode. Barcode gap and resolving power were increased when ITS was incorporated into the rbcL. The barcode sequences were deposited to the National Center for Biotechnology Information (NCBI) database for public use." |
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| Language: | Korean |
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| References: | 21 |
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| Note: | Abstract appears in English only
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Lee, J., C.-S. Kim, and I.-Y. Lee. 2015. Molecular identification of Pooideae, Poaceae in Korea. (In Korean) Weed and Turfgrass Science. 4(1):p. 18-25. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=265193 |
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| DOI: 10.5660/WTS.2015.4.1.18 |
| Web URL(s):
http://ocean.kisti.re.kr/downfile/volume/ksws/JDHHCQ/2015/v4n1/JDHHCQ_2015_v4n1_18.pdf
Last checked: 09/24/2015
Requires: PDF Reader |
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