Full TGIF Record # 267554
Item 1 of 1
Web URL(s):http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-105-11-S4.1#page=69
    Last checked: 12/14/2015
    Requires: PDF Reader
    Notes: Item is within a single large file
Publication Type:
i
Report
Author(s):Karakkat, B.; Jackson, V.; Koch, P.
Author Affiliation:University of Wisconsin-Madison, Madison, WI
Title:Molecular detection of rust fungi on turfgrass
Section:2015 APS Annual Meeting abstracts of presentations
Other records with the "2015 APS Annual Meeting abstracts of presentations" Section
Meeting Info.:Pasadena, California: August 1-5, 2015
Source:Phytopathology. Vol. 105, No. 11S, November 2015, p. S4.69.
Publishing Information:Lancaster, Pennsylvania: The Society Intelligencer Printing Company for The American Phytopathological Society
# of Pages:1
Keywords:TIC Keywords: Analytical methods; Disease distribution; Disease evaluation; Disease identification; Disease severity; Polymerase chain reaction; Puccinia coronata var. coronata; Puccinia graminis subsp. graminis; Rusts
Abstract/Contents:"Rust is one of the main diseases of turfgrass in Wisconsin and across the country. Historically, the two primary rust diseases on cool-season turfgrasses have been crown rust [CR] (Puccinia coronata) and stem rust [SR] (Puccinia graminis). Increasing rust severity has been documented in the past 10 years, and recent research indicates it may be due to increasing activity of CR on a wider array of turfgrass species. We are developing a Quantitative polymerase chain reaction (QPCR) detection method to boost this screening process so that the identification is more reliable and faster than only microscopic observation of urediniospores and teliospores. Initial attempts at extraction of genomic DNA from rust infected leaf samples gave only a few positive results in the traditional PCR mainly because genomic DNA from uredinia were only present in trace amounts. However, more consistent positive results were obtained when QPCR was employed with the same genomic DNA using sequence specific fluorescent probes of P. coronata and P. graminis. By conducting this study, we aim to test over 400 rust samples collected from different turf plots from around the Midwest in 2013 and 2014 and report the percentage of each Puccinia species observed."
Language:English
References:0
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Karakkat, B., V. Jackson, and P. Koch. 2015. Molecular detection of rust fungi on turfgrass. Phytopathology. 105(11S):p. S4.69.
Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=267554
If there are problems with this record, send us feedback about record 267554.
Choices for finding the above item:
Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-105-11-S4.1#page=69
    Last checked: 12/14/2015
    Requires: PDF Reader
    Notes: Item is within a single large file
Find Item @ MSU
MSU catalog number: b2219736a
Find from within TIC:
   Digitally in TIC by file name: phytp2015novpres
Request through your local library's inter-library loan service (bring or send a copy of this TGIF record)