| |
Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-105-11-S4.1#page=69 Last checked: 12/14/2015 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Report |
Author(s): | Karakkat, B.;
Jackson, V.;
Koch, P. |
Author Affiliation: | University of Wisconsin-Madison, Madison, WI |
Title: | Molecular detection of rust fungi on turfgrass |
Section: | 2015 APS Annual Meeting abstracts of presentations Other records with the "2015 APS Annual Meeting abstracts of presentations" Section
|
Meeting Info.: | Pasadena, California: August 1-5, 2015 |
Source: | Phytopathology. Vol. 105, No. 11S, November 2015, p. S4.69. |
Publishing Information: | Lancaster, Pennsylvania: The Society Intelligencer Printing Company for The American Phytopathological Society |
# of Pages: | 1 |
Keywords: | TIC Keywords: Analytical methods; Disease distribution; Disease evaluation; Disease identification; Disease severity; Polymerase chain reaction; Puccinia coronata var. coronata; Puccinia graminis subsp. graminis; Rusts
|
Abstract/Contents: | "Rust is one of the main diseases of turfgrass in Wisconsin and across the country. Historically, the two primary rust diseases on cool-season turfgrasses have been crown rust [CR] (Puccinia coronata) and stem rust [SR] (Puccinia graminis). Increasing rust severity has been documented in the past 10 years, and recent research indicates it may be due to increasing activity of CR on a wider array of turfgrass species. We are developing a Quantitative polymerase chain reaction (QPCR) detection method to boost this screening process so that the identification is more reliable and faster than only microscopic observation of urediniospores and teliospores. Initial attempts at extraction of genomic DNA from rust infected leaf samples gave only a few positive results in the traditional PCR mainly because genomic DNA from uredinia were only present in trace amounts. However, more consistent positive results were obtained when QPCR was employed with the same genomic DNA using sequence specific fluorescent probes of P. coronata and P. graminis. By conducting this study, we aim to test over 400 rust samples collected from different turf plots from around the Midwest in 2013 and 2014 and report the percentage of each Puccinia species observed." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Karakkat, B., V. Jackson, and P. Koch. 2015. Molecular detection of rust fungi on turfgrass. Phytopathology. 105(11S):p. S4.69. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=267554 |
| If there are problems with this record, send us feedback about record 267554. |
| Choices for finding the above item: |
| Web URL(s): http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-105-11-S4.1#page=69 Last checked: 12/14/2015 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: b2219736a |
| Find from within TIC: Digitally in TIC by file name: phytp2015novpres |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |