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Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-105-11-S4.1#page=124 Last checked: 12/14/2015 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Report |
Author(s): | Scott, J. C.;
Kaur, N.;
Alderman, S. C.;
Walenta, D. L.;
Hamm, P. B.;
Frost, K. E.;
Dung, J. K. S. |
Author Affiliation: | Scott and Dung: Oregon State University, Madras; Kaur, Hamm, and Frost: Oregon State University, Hermiston; Alderman: USDA-ARS NFSPRC, Corvallis; Walenta: Oregon State University, La Grande, OR |
Title: | Molecular differentiation of Claviceps isolates from Kentucky bluegrass and perennial ryegrass in Oregon and Washington |
Section: | 2015 APS Annual Meeting abstracts of presentations Other records with the "2015 APS Annual Meeting abstracts of presentations" Section
|
Meeting Info.: | Pasadena, California: August 1-5, 2015 |
Source: | Phytopathology. Vol. 105, No. 11S, November 2015, p. S4.124-S4.125. |
Publishing Information: | Lancaster, Pennsylvania: The Society Intelligencer Printing Company for The American Phytopathological Society |
# of Pages: | 2 |
Keywords: | TIC Keywords: Claviceps purpurea; Disease evaluation; Ecotypes; Ergot; Isolation technique; Lolium perenne; Mating type; Poa pratensis
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Geographic Terms: | Oregon; Washington |
Abstract/Contents: | "Ergot, caused by the fungus Claviceps purpurea, is an economically important seed replacement disease of Kentucky bluegrass (Poa pratensis) and perennial ryegrass (Lolium perenne) seed crops. Recently, C. purpurea ecotypes G1, G2, and G3 were defined as three separate species: C. purpurea sensu stricto, C. humidiphila, and C. spartinae, respectively. A collection of 25 Claviceps isolates collected from P. pratensis and L. perenne in Oregon (OR) and Washington (WA) between 2010 and 2014 were characterized using RAPD patterns, ITS sequences, and mating type. Three isolates from P. pratensis and nine isolates from L. perenne collected in OR exhibited RAPD patterns corresponding to C. purpurea and ITS sequences grouped the isolates with ITS sequences from C. purpurea previously deposited in GenBank. Nine isolates from P. pratensis collected in WA produced RAPD patterns and ITS sequences corresponding to C. humidiphila; however, one isolate each from OR and WA exhibited RAPD patterns associated with C. humidiphila but ITS sequences grouped them with C. purpurea. One isolate each from P. pratensis and L. perenne produced RAPD patterns matching C. purpurea but formed their own subclade using ITS sequences. Mating type PCR assays detected the MAT1-1- 1 gene in 18 isolates and the MAT1-2-1 gene in two isolates; five isolates possessed both genes. This is the first study to identify different Claviceps species infecting P. pratensis and L. perenne seed crops in OR and WA." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Scott, J. C., N. Kaur, S. C. Alderman, D. L. Walenta, P. B. Hamm, K. E. Frost, et al. 2015. Molecular differentiation of Claviceps isolates from Kentucky bluegrass and perennial ryegrass in Oregon and Washington. Phytopathology. 105(11S):p. S4.124-S4.125. |
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| Web URL(s): http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-105-11-S4.1#page=124 Last checked: 12/14/2015 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: b2219736a |
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