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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-105-11-S4.1#page=142
Last checked: 12/14/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Author(s): | Villari, C.;
Mahaffee, W. F.;
Mitchell, T. K.;
Pedley, K. F.;
Pieck, M.;
Peduto Hand, F. |
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| Author Affiliation: | Villari, Mitchell, and Peduto Hand; Department of Plant Pathology, The Ohio State University, Columbus, OH; Mahaffee: USDA ARS, Corvallis, OR; Pedley and Pieck: USDA ARS, Fort Detrick, MD |
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| Title: | Development of a quantitative loop-mediated isothermal amplification assay to detect Magnaporthe oryzae airborne inoculum in turf ecosystems |
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| Section: | 2015 APS Annual Meeting abstracts of presentations
Other records with the "2015 APS Annual Meeting abstracts of presentations" Section
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| Meeting Info.: | Pasadena, California: August 1-5, 2015 |
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| Source: | Phytopathology. Vol. 105, No. 11S, November 2015, p. S4.142-S4.143. |
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| Publishing Information: | Lancaster, Pennsylvania: The Society Intelligencer Printing Company for The American Phytopathological Society |
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| # of Pages: | 2 |
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| Keywords: | TIC Keywords: DNA amplification; Disease development; Disease evaluation; Ecosystems; Gray leaf spot; Pyricularia grisea; Quantitative techniques
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| Abstract/Contents: | "Grey Leaf Spot (GLS) is a detrimental disease of perennial ryegrass caused by a host-specialized form of Magnaporthe oryzae (Lolium pathotype, moL). In order to improve turf management, a quantitative loop-mediated isothermal amplification (LAMP) assay coupled with a simple spore trap is being developed to monitor GLS airborne inoculum. LAMP has been shown to be suitable for implementation by practitioners to initiate and time fungicide applications in vineyards. Since the most common conserved regions such as ITS, LSU and Calmodulin lack the needed heterogeneity among the different host-specialized forms of M. oryzae, LAMP primers were designed to a nonannotated genomic DNA region unique to moL. Reactions were performed on a Real-Time PCR Detection System and optimized to provide a response in approximately 30 minutes. Quantification of the inoculum was obtained by comparison with a standard curve developed using 6 independent serial spore dilutions. The LAMP assay was specific to the host-specialized forms of M. oryzae and was able to detect greater than 500 spores, indicating that it is capable of detecting the pathogen at the very beginning of the disease epidemic. The implementation of this assay could be a useful decision support tool to guide initiation and timing of fungicide applications for GLS management." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Villari, C., W. F. Mahaffee, T. K. Mitchell, K. F. Pedley, M. Pieck, and F. Peduto Hand. 2015. Development of a quantitative loop-mediated isothermal amplification assay to detect Magnaporthe oryzae airborne inoculum in turf ecosystems. Phytopathology. 105(11S):p. S4.142-S4.143. |
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| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-105-11-S4.1#page=142
Last checked: 12/14/2015
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: b2219736a |
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Digitally in TIC by file name: phytp2015novpres |
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