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| DOI: | 10.3852/13-006 |
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| Web URL(s): | http://www.mycologia.org/content/106/1/163.full.pdf
Last checked: 02/03/2016
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http://www.mycologia.org/content/106/1/163.full
Last checked: 02/03/2016
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Amaradasa, Bimal S.;
Lakshman, Dilip;
Horvath, Brandon J.;
Amundsen, Keenan L. |
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| Author Affiliation: | Amaradasa and Amundsen: Department of Agronomy and Horticulture, University of Nebraska at Lincoln, Lincoln, Nebraska; Lakshman: Floral and Nursery Plants Research Unit, Beltsville Agricultural Research Center-West, Beltsville, Maryland; Horvath: Department of Plant Sciences, University of Tennessee, Knoxville, Tennessee |
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| Title: | Development of SCAR markers and UP-PCR cross-hybridization method for specific detection of four major subgroups of Rhizoctonia from infected turfgrasses |
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| Section: | Techniques
Other records with the "Techniques" Section
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| Source: | Mycologia. Vol. 106, No. 1, January/February 2014, p. 163-172. |
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| Publishing Information: | Lancaster, Pennsylvania: New Era Print Co. for the New York Botanical Garden |
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| # of Pages: | 10 |
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| Related Web URL: | http://www.mycologia.org/content/106/1/163.abstract
Last checked: 02/03/2016
Notes: Abstract only |
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| Keywords: | TIC Keywords: Cool season turfgrasses; Hybridization; Patch diseases; Polymerase chain reaction; Rhizoctonia; Rhizoctonia blight; Waitea circinata var. circinata; Waitea circinata var. zeae
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| Abstract/Contents: | "A rapid identification assay for Waitea circinata (anamorph: Rhizoctonia spp.) varieties zeae and circinata causing patch diseases on turfgrasses was developed based on the universally primed PCR (UP-PCR) products cross-blot hybridization. Tester isolates belonging to the two varieties of W. circinata were amplified with a single UP primer L21, which generated multiple DNA fragments for each variety. Probes were prepared with UP-PCR products of each tester isolate by labeling with digoxigenin. Fieldcollected W. circinata isolates and representative isolates of different R. solani anastomosis groups (AG) and AG subgroups were amplified with L21, immobilized on nylon membrane and cross hybridized with the two probes. Isolates within a W. circinata variety cross-hybridized strongly, while non-homologous isolates did not cross-hybridize or did so weakly. Closely related W. circinata varieties zeae and circinata were clearly distinguished with this assay. Sequence-characterized amplified region (SCAR) markers also were developed from UP-PCR products to identify isolates of Thanatephorus cucumeris (anamorph: R. solani) AG 1-IB and AG 2-2IIIB. These two AGs are commonly isolated from diseased, cool-season turfgrasses. The specific SCAR markers that were developed could differentiate isolates of AG 1-IB or AG 2-2IIIB groups. These SCAR markers did not amplify a product from genomic DNA of nontarget isolates of Rhizoctonia. The specificities and sensitivities of the SCAR primers were tested on total DNA extracted from several field-grown, cool-season turf species having severe brown-patch symptoms. First, the leaf samples from diseased turf species were tested for the anastomosis groups of the causal pathogen, and thereafter the total DNA was amplified with the specific primers. The specific primers were sensitive and unique enough to produce a band from total DNA of diseased turfgrasses infected with either AG 1-IB or AG 2-2IIIB." |
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| Language: | English |
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| References: | 35 |
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| Note: | Pictures, b/w
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Amaradasa, B. S., D. Lakshman, B. J. Horvath, and K. L. Amundsen. 2014. Development of SCAR markers and UP-PCR cross-hybridization method for specific detection of four major subgroups of Rhizoctonia from infected turfgrasses. Mycologia. 106(1):p. 163-172. |
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| DOI: 10.3852/13-006 |
| Web URL(s):
http://www.mycologia.org/content/106/1/163.full.pdf
Last checked: 02/03/2016
Requires: PDF Reader
Access conditions: Item is within a limited-access website
http://www.mycologia.org/content/106/1/163.full
Last checked: 02/03/2016
Access conditions: Item is within a limited-access website |
| 
MSU catalog number: b2214983
MSU catalog number: b5343430 |
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