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DOI: | 10.3732/apps.1400093 |
Web URL(s): | http://www.bioone.org/doi/full/10.3732/apps.1400093 Last checked: 03/03/2016 http://www.bioone.org/doi/pdf/10.3732/apps.1400093 Last checked: 03/03/2016 Requires: PDF Reader |
Publication Type:
| Refereed |
Author(s): | von CrƤutlein, Maria;
Korpelainen, Helena;
Helander, Marjo;
Ćhberg, Annika;
Saikkonen, Kari |
Author Affiliation: | von CrƤutlein, Helander, and Saikkonen: MTT Agrifood Research Finland, Plant Production Research, Tietotie, Jokioinen; Korpelainen and Ćhberg: Department of Agricultural Sciences, University of Helsinki, Latokartanonkaari, University of Helsinki; Helander: Department of Biology, University of Turku, Turku, Finland |
Title: | Development and characterization of nuclear microsatellite markers in the endophytic fungus Epichloƫ festucae (Clavicipitaceae) |
Section: | Primer notes Other records with the "Primer notes" Section
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Source: | Applications in Plant Sciences. Vol. 2, No. 12, December 2014, p. 1400093. |
Publishing Information: | St. Louis, Missouri: Botanical Society of America |
# of Pages: | 4 |
Keywords: | TIC Keywords: Breeding aims; Comparisons; Cultivar improvement; Endophytic fungi; Epichloe festucae; Genetic analysis; Genetic markers; Haploids; Microsatellites; Polymorphism; Population dynamics; Regional variation
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Abstract/Contents: | "Premise of the study: Microsatellite primers were developed for the endophytic fungus Epichloƫ festucae, which is symbiotic with Festuca rubra, to study the population genetics of the species and to compare population structures between E. festucae and its host F. rubra. Methods and Results: We developed 14 polymorphic markers using the unplaced genomic scaffold sequences of E. festucae from GenBank. The number of alleles per locus (A) varied from four to 16, and unbiased haploid diversity (h) was 0.717 in eight populations located in the Faroe Islands, Finland, and Spain. The Spanish populations possessed a higher number of alleles and haploid diversity (on average A = 5.1 and h = 0.591, respectively) compared to northern populations (on average A = 1.5 and h = 0.199, respectively). Conclusions: These polymorphic markers will be used by grass breeders for uses including the improvement of commercial turfgrass cultivars, and by population geneticists to study different species of the Epichloƫ genus." |
Language: | English |
References: | 15 |
Note: | Tables |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): von CrƤutlein, M., H. Korpelainen, A. Ćhberg, and K. Saikkonen. 2014. Development and characterization of nuclear microsatellite markers in the endophytic fungus EpichloĆ« festucae (Clavicipitaceae). Appl. Plant Sci. 2(12):p. 1400093. |
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| DOI: 10.3732/apps.1400093 |
| Web URL(s): http://www.bioone.org/doi/full/10.3732/apps.1400093 Last checked: 03/03/2016 http://www.bioone.org/doi/pdf/10.3732/apps.1400093 Last checked: 03/03/2016 Requires: PDF Reader |
| MSU catalog number: b9889928 |
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