Full TGIF Record # 269520
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Web URL(s):http://aijbnet.com/journals/aijb/Vol_2_No_1_March_2014/1.pdf
    Last checked: 03/04/2016
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Publication Type:
i
Refereed
Author(s):Amaradasa, Bimal S.; Amundsen, Keenan
Author Affiliation:PhD, Department of Agronomy and Horticulture, University of Nebraska-Lincoln, NE
Title:Expression of leaf spot induced resistance gene-like sequences in buffalograss
Source:American International Journal of Biology. Vol. 2, No. 1, March 2014, p. 1-19.
Publishing Information:Madison, Wisconsin: American Research Institute for Policy Development
# of Pages:19
Keywords:TIC Keywords: Bouteloua dactyloides; Cultivar evaluation; Cultivar profile; Curvularia inaequalis; Disease resistance; Genetic analysis; Genetic resistance; Leaf spot; Reverse transcriptase polymerase chain reaction
Abstract/Contents:"American buffalograss [Buchloë dactyloides (Nutt.) Engelm. syn. Bouteloua dactyloides (Nutt.) Columbus] is a drought, heat and cold tolerant United States native turfgrass species. Leaf spot disease caused by Curvularia inaequalis negatively impacts buffalograss visual quality. Seven candidate resistance gene-like sequenceshave been previously identified in buffalograss based on similarity to known resistance genes. Here, the expression of these seven genes were evaluated in two leaf spot susceptible (Prestige and NE-BFG-7-3453-50) and two resistant (95-55 and NEBFG- 7-3459-17) buffalograss lines when challenged with C. Inaequalis. Real-time quantitative PCR assays were used to detect expression of the sequences on each buffalograss line. Four of the sequences were expressed in all buffalograss lines. One sequence had higher expression in both resistant lines and another had higher expression in 95-55 when challenged with the pathogen. Compared to uninoculated controls, inoculated susceptible lines did not show a change in expression of resistance gene-like sequences. Inoculated resistant lines had higher expression of the tested resistance gene-like sequences than the susceptible lines. Expression of these genes was elevated in uninoculated plants and may contribute to the innate immunity to leaf spot disease of the resistant lines."
Language:English
References:22
Note:Tables
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Amaradasa, B. S., and K. Amundsen. 2014. Expression of leaf spot induced resistance gene-like sequences in buffalograss. American International Journal of Biology. 2(1):p. 1-19.
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    Last checked: 03/04/2016
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