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| DOI: | 10.3852/12-368 |
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| Web URL(s): | http://www.mycologia.org/content/105/5/1190.full
Last checked: 03/17/2016
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http://www.mycologia.org/content/105/5/1190.full.pdf+html
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| Publication Type:
| Refereed |
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| Author(s): | Amaradasa, B. S.;
Horvath, B. J.;
Lakshman, D. K.;
Warnke, S. E. |
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| Author Affiliation: | Amaradasa: Department of Agronomy and Horticulture, University of Nebraska, Lincoln, Nebraska; Horvath: Department of Plant Sciences, University of Tennessee, Knoxville, Tennessee; Lakshman and Warnke: Floral and Nursery Plants Research Unit, Beltsville Agricultural Research Center-West, Beltsville, Maryland |
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| Title: | DNA fingerprinting and anastomosis grouping reveal similar genetic diversity in Rhizoctonia species infecting turfgrasses in the transition zone of USA |
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| Section: | Plant pathogens
Other records with the "Plant pathogens" Section
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| Source: | Mycologia. Vol. 105, No. 5, September/October 2013, p. 1190-1201. |
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| Publishing Information: | Lancaster, Pennsylvania: New Era Print Co. for the New York Botanical Garden |
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| # of Pages: | 12 |
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| Related Web URL: | http://www.mycologia.org/content/105/5/1190.abstract
Last checked: 03/17/2016
Notes: Abstract only |
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| Keywords: | TIC Keywords: Anastomosis reactions; DNA amplification; Disease development; Disease identification; Disease control; Evaluative methods; Genetic diversity; Rhizoctonia; Rhizoctonia blight; Rhizoctonia solani; Thanatephorus cucumeris; Transition zone; Waitea circinata var. circinata; Waitea circinata var. oryzae; Waitea circinata var. zeae
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| Abstract/Contents: | "Rhizoctonia blight is a common and serious disease of many turfgrass species. The most widespread causal agent, Thanatephorus cucumeris (anamorph: R. solani), consists of several genetically different subpopulations. In addition, Waitea circinata varieties zeae, oryzae and circinata (anamorph: Rhizoctonias pp.) also can cause the disease. Accurate identification of the causal pathogen is important for effective management of the disease. It is challenging to distinguish the specific causal pathogen based on disease symptoms or macroscopic and microscopic morphology. Traditional methods such as anastomosis reactions with tester isolates are time consuming and sometimes difficult to interpret. In the present study universally primed PCR (UP-PCR) fingerprinting was used to assess genetic diversity of Rhizoctonia spp. infecting turfgrasses. Eighty-four Rhizoctonia isolates were sampled from diseased turfgrass leaves from seven distinct geographic areas in Virginia and Maryland. Rhizoctonia isolates were characterized by ribosomal DNA internal transcribed spacer (rDNA-ITS) region and UP-PCR. The isolates formed seven clusters based on ITS sequences analysis and unweighted pair group method with arithmetic mean (UPGMA) clustering of UP-PCR markers, which corresponded well with anastomosis groups (AGs) of the isolates. Isolates of R. solani AG 1-IB (n = 18), AG 2-2IIIB (n = 30) and AG 5 (n = 1) clustered separately. Waitea circinata var. zeae (n = 9) and var. circinata (n = 4) grouped separately. A cluster of six isolates of Waitea (UWC) did not fall into any known Waitea variety. The binucleate Rhizoctonia-like fungi (BNR) (n = 16) clustered into two groups. Rhizoctonia solani AG 2-2IIIB was the most dominant pathogen in this study, followed by AG 1-IB. There was no relationship between the geographic origin of the isolates and clustering of isolates based on the genetic associations. To our knowledge this is the first time UP-PCR was used to characterize Rhizoctonia, Waitea and Ceratobasidium isolates to their infra-species level." |
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| Language: | English |
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| References: | 40 |
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| Note: | Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Amaradasa, B. S., B. J. Horvath, D. K. Lakshman, and S. E. Warnke. 2013. DNA fingerprinting and anastomosis grouping reveal similar genetic diversity in Rhizoctonia species infecting turfgrasses in the transition zone of USA. Mycologia. 105(5):p. 1190-1201. |
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| DOI: 10.3852/12-368 |
| Web URL(s):
http://www.mycologia.org/content/105/5/1190.full
Last checked: 03/17/2016
Access conditions: Item is within a limited-access website
http://www.mycologia.org/content/105/5/1190.full.pdf+html
Last checked: 03/17/2016
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: b2214983
MSU catalog number: b5343430 |
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