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| Web URL(s): | http://manu40.magtech.com.cn/Jweb_cdxb/CN/article/downloadArticleFile.do?attachType=PDF&id=1626
Last checked: 04/22/2016
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Author(s): | Li, Wei;
Zhang, Na;
Li, Ren;
Zhao, Bing;
Guo, Yang-dong |
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| Author Affiliation: | College of Agriculture and Biotechnology, China Agricultural University, Beijing, China |
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| Title: | Establishment of high efficient regeneration system of perennial ryegrass and PEPC gene transformation mediated by Agrobacterium tumafaciens |
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| Column Name: | Scientific note
Other records with the "Scientific note" Column
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| Source: | [Cao Di Sue Bao] [Acta Agrestia Sinica]. Vol. 20, No. 4, July 2012, p. 747-752. |
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| Publishing Information: | [Beijing, China]: [Zhongguo Xue Shu Qi Kan (Guang Pan Ban) Dian Zi Za Zhi She] |
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| # of Pages: | 6 |
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| Keywords: | TIC Keywords: Agrobacterium tumefaciens; Breeding aims; Calli; Evaluations; Genetic transformation; Lolium perenne; Regeneration
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| Abstract/Contents: | "In order to establish a highly efficient regeneration and transformation system of perennial ryegrass (Lolium perenne L.), its mature seeds were used as explants. Result showed that the best induction rate was 61.33%. Callus differentiated after 2~3 generation. The optimum medium for callus differentiation was MS+6-BA at 1.0 mg-1 with 61.33% regeneration rate. The best medium for rooting was 1/2 MS+NAA at 0.5 mg-1 and the ratio of rooting was 100%. Based on this regeneration system, the embryonic callus cells were used for Agrobacterium-mediated transformation. Transformed tissues were regenerated with media containing hyg as a selective solvent. PCR and Real-Time PCR analysis of transformants showed that PEPC gene was successfully integrated into the genome of perennial ryegrass and transformation rate was 8.67%." |
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| Language: | Chinese |
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| References: | 23 |
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| Note: | Abstract also appears in English
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Li, W., N. Zhang, R. Li, B. Zhao, and Y.-D. Guo. 2012. Establishment of high efficient regeneration system of perennial ryegrass and PEPC gene transformation mediated by Agrobacterium tumafaciens. (In Chinese) [Cao Di Sue Bao] [Acta Agrestia Sinica]. 20(4):p. 747-752. |
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| Web URL(s):
http://manu40.magtech.com.cn/Jweb_cdxb/CN/article/downloadArticleFile.do?attachType=PDF&id=1626
Last checked: 04/22/2016
Requires: PDF Reader |
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