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| Web URL(s): | http://cykx.lzu.edu.cn//EN/article/downloadArticleFile.do?attachType=PDF&id=11144
Last checked: 04/13/2016
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Author(s): | Niu, Kui-ju;
Yu, Ling;
Li, Yu-zhu;
Ma, Hui-ling |
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| Author Affiliation: | Pratacultural College, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Sino-U.S. Centers for Grazingland Ecosystem Sustainability, Lanzhou, China |
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| Title: | Protoplast isolation and culture of wild Kentucky Bluegrass in Gansu |
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| Source: | [Caoye Kexue] [Pratacultural Science]. Vol. 32, No. 6, June 15 2015, p. 927-934. |
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| Publishing Information: | [Lanzhou Shi, China]: ["Cao Ye Ke Xue" Bian Jibu] |
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| # of Pages: | 8 |
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| Keywords: | TIC Keywords: Cultivar evaluation; Cultivar variation; Embryogenesis; Enzymes; Isolation technique; Poa pratensis; Protoplasts
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| Cultivar Names: | Longxi; Dingxi |
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| Geographic Terms: | Gansu, China |
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| Abstract/Contents: | "In the present study, the conditions of protoplast isolation and culture were explored using embryogenic callus from wild Kentucky Bluegrass variety Longxi (LX) and Dingxi (DX). The results showed that the optimal conditions for protoplast isolation was enzyme composition including 1.5% Cellulase R-10+0.5% Pectolase Y-23+1.0% Macerozyme R-10 + 0.3% Driselase with 16 h digestion. The optimal mannitol concentration was 0.6 mol 路 L-1 for LX protoplast and 0.5 mol 路 L-1 for DX protoplast. The highest yield of protoplasts was 6.59^D#x 106 number 路 g-1 for LX and 5.95^D#x106 number 路 g-1 for DX. After purification, DX protoplasts were cultured in KM8P liquid medium. With 3.0^D#x105 number 路 m L-1 plating density and 1.0 mg 路 L-1 2, 4-D, DX protoplasts had highest cell division frequency of 9.56% and plating efficiency of 4.62%." |
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| Language: | Chinese |
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| References: | 21 |
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| Note: | Abstract also appears in English
Pictures, color
Tables
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Niu, K.-j., L. Yu, Y.-z. Li, and H.-l. Ma. 2015. Protoplast isolation and culture of wild Kentucky Bluegrass in Gansu. (In Chinese) [Caoye Kexue] [Pratacultural Science]. 32(6):p. 927-934. |
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| Web URL(s):
http://cykx.lzu.edu.cn//EN/article/downloadArticleFile.do?attachType=PDF&id=11144
Last checked: 04/13/2016
Requires: PDF Reader |
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MSU catalog number: b10275836 |
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